Insect-XPRESS™ is a popular protein-free medium designed for use on invertebrate cell lines including Sf9, Sf21, and Hi-5
Insect-XPRESS™ Protein-free Insect Cell Medium is a formulation designed to support the growth of insect cell lines derived from Spodoptera frugiperda (Sf9 and Sf21). Cell densities in excess of 8.3 × 106
cells/ml can be achieved with suspension cultures of Sf9 cells using Insect-XPRESS™ Medium and an excess of oxygen.
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Insect-XPRESS™ Medium was developed to support the growth of invertebrate cell lines, derived from the fall army worm, Spodoptera frugiperda mainly Sf9 and Sf21. The Insect-XPRESS™ Medium formulation is protein-free (all components are less than or equal to 10,000 mwt.) and has been designed to support the growth of insect cells under attachmentdependent or attachment-independent conditions.
Cell densities in excess of 8.3 + 106 cells/mL have been achieved with suspension cultures of Sf9 cells using Insect-XPRESS™ Medium and an excess of oxygen. Insect-XPRESS™ Medium may also be used for stationary monolayer cultures and shakeflask cultures. The Insect-XPRESS™ 1X liquid formulation contains L-glutamine and supports the production of recombinant proteins by cells infected with viral vectors such as the Baculovirus. Insect-XPRESS™ Medium may be used as a base for the cryopreservation of insect cells. We recommend that the Insect-XPRESS™ Medium liquid be mixed 50:50 with Cryoprotective Medium prior to cryopreservation.
Serum-free media and reagents have a wide range of applications, including production of monoclonal antibodies, viral antigens, and recombinant proteins using a variety of mammalian and invertebrate cell lines. There are numerous advantages associated with the use of serum-free media formulations.
- No need for serum qualification
- Increased definition
- Simplified purification and downstream processing
- Better control over the physiological condition of cultures
- Ability to optimize formulations for specific cell types