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Vivaflow 200, 10,000 MWCO PES

0.5 - 5 l

  • Plug and play laboratory cross flow cassette
  • Re-usable
  • Optimal for cell culture supernatant and virus concentration
  • High flow rate and recovery

Order Number:  VF20P0

Availability:  On Request

Vivaflow® 200 is a ready to use and reusable laboratory crossflow cassette for concentrating up to 5 l samples. The membrane surface per device is 200 cm². One unit comes with all necessary accessories for running the device with a laboratory pump and a size 16 pump head. For speeding up the concentration, two cassettes can be run simultaneously.

Vivaflow® 200 is optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration e.g. in fish breeding facilities. This economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market. The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single 200 cm2 module typically concentrates 1 l by a factor of 50 in less than 30 min. Alternatively, two Vivaflow® 200 modules can be used in parallel for concentration of 5 l in under 75 minutes. Near total recovery of the concentrate is achieved with a single final rinse. Vivaflow® 200 can be easily cleaned and re-used several times. All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package for using a single cassette. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange.

Equipment required

The standard Vivaflow® 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).

Equipment required

The standard Vivaflow® 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).

Running 2 Vivaflow® 200 cassettes in parallel

For speeding up the concentration, two Vivaflow® 200 cassettes can easily be connected using a Y connector which is preassembeled with the correct tubing sizes and fittings (size 16 tubing for connecting the two Vivaflow® 200 units, size 15 tubing for the pump). Please note that you need a size 15 pump head for running 2 Vivaflows® in parallel.

  • High flow rate and recovery
  • Clear housing for process monitoring
  • Ready to use single cassettes
  • Easy set up of 2 cassettes for use in parallel
  • Re-usable product

Performance Characteristics

  Time to concentrate up to 20x (min.) at 3 bar
inlet pressure, 20°C
 Start volumeAverage fluxRecovery % 
 1 litreml/minDirect25 ml rinse
Lysozyme 0.25 mg/ml (14,000 MW)
2,000 MWCO Hydrosart160697 %>99 %
3,000 MWCO PES180597 %>99 %
BSA 1.0 mg/ml (66,000 MW)
5,000 MWCO PES293398 %>99 %
5,000 MWCO Hydrosart701498 %>99 %
10,000 MWCO PES234196 %>99 %
10,000 MWCO Hydrosart352798 %>99 %
30,000 MWCO PES253896 %99 %
30,000 MWCO Hydrosart204896 %>99 %
50,000 MWCO PES224396 %98 %
γ Globulins 1.0 mg/ml (average 160,000 MW)
100,000 MWCO PES541896 %99 %
100,000 MWCO RC452196 %99 %
Yeast 1.0 mg/ml (S. Cerevisiae)
0.2 μm PES118692 %98 %
Dilute solute concentration, start volume 1 litre at 3 bar, 10,000 MWCO PES
BSA 0.001 mg/ml185290 %98 %
BSA 0.01 mg/ml204792 %98 %
BSA 0.1 mg/ml214594 %99 %
Start volume 5 litres (two VF200 in parallel at 3 bar) 10,000 MWCO PES
BSA 1.0 mg/ml (66,000 MW)677097 %>99 %

Application Note

Sartorius Ultrafiltration Products in the Preparation of Biological and Medical Nanocarriers – a Short Review
en 133.1 kB
Vivaflow® and Vivaspin® Workflow in Protein Research Laboratories
en 827.9 kB
The Measurement of Soluble (< 10 kDa) Trace Metals in seawater by the Vivaflow 50
en 269.6 kB

Brochure

Ultrafiltration Family
en 1.6 MB

Catalog

Laboratory Filtration Products (US and Canada)
en 9.6 MB

Datasheet

Vivaflow® 50 | 50R | 200 — Unique, “Plug & Play” Laboratory Crossflow Devices
en 677.1 kB

User Manual

Vivaflow 50|200
fr 543.1 kB
Vivaflow 50 | 50R | 200
en 731.4 kB

Product properties

  • MWCO
    10,000 Daltons
  • Width
    38 mm
  • Volume Range
    500 – 5,000 mL
  • Max. operating pressure
    4 bar
  • Primary Product Type
    Ultrafiltration Devices
  • Membrane material
    Polyethersulfone (PES)
  • Membrane
    Polyethersulfone (PES)
  • Tubing
    PVC (medical grade)
  • Material o-ring | seal
    Silicone (SI)

Technical attributes

  • Electrical / battery item
    No
  • Animal Components
    No
  • Housing material
    Acrylic
  • Pressure indicator
    Polypropylene, SS spring
  • Flow Channel
    Acrylic
  • Flow Restrictor
    Polypropylene (PP)
  • Maximum Pressure
    4 bar
  • Overall length
    127 mm
  • Hold-up volume
    5.3 mL
  • Active Membrane Area
    200 cm²
  • Overall height
    138 mm
  • Max. temperature
    60° C
  • Non-recoverable Hold-up
    < 2 mL
  • Channel width
    10 mm
  • Minimum Recirculation Volume
    < 20 mL
  • Channel height
    0.4 mm
  • Pump Flow
    200 – 400 mL/min
  • Certification
    None
  • Application
    Protein Concentration
  • Area of application
    Buffer Exchange` Protein/DNA Concentration
  • Market
    Research
  • Device type
    Tangential / Crossflow Concentrator
  • Sample Type
    Protein` Peptide` Nanoparticles` Virus
  • Fittings
    Nylon (NY)
  • Membrane support
    Polypropylene (PP)