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RoboColumn HEA HyperCel 200 µL

HEA HyperCel is a mixed-mode resin with a less hydrophobic hexylamine ligand allowing protein binding at a moderate salt concentration under neutral pH. Pre-packed columns simplify resin screening and selection, and are ideal for process step optimization. The RoboColumn format is especially suited to high throughput process development (HTPD).

Item no.:  SR2HEA


HEA HyperCel resin is an industry-scalable chromatography resin designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a “mixed-mode” mechanism, with a hexylamine ligand, its behavior is based on a combination of electrostatic and hydrophobic interactions of the proteins with the ligands. HEA HyperCel resin provides unique selectivities, different from those given by ion exchange or conventional HIC (hydrophobic interaction chromatography).


Features and Benefits

- High mechanical and chemical stability
- Suitable for large-scale chromatography in a biopharmaceutical environment
- Cross-linked cellulose base matrix


- Protein purification at low ionic strength by direct hydrophobic capture
- Separation of challenging mixtures, e.g. protein isoforms or proteins with very close pIs
- Orthogonal separation mechanism to ion exchange or HIC


Available Formats for HEA HyperCel

Pack SizeOrder Code
25 mL20250-026
100 mL20250-033
1 L20250-041
5 L20250-042
10 L20250-056


As pre-packed columns:

Pack SizeOrder Code
1 mL PRC Prepacked Column, 5 mm ID x 50 mmPRC05X050HEAHCEL
5 mL PRC Prepacked Column, 8 mm ID x 100 mmPRC08X100HEAHCEL
RoboColumn HEA HyperCel 200 μL, row of 8SR2HEA
RoboColumn HEA HyperCel 600 μL, row of 8SR6HEA



HEA and PPA HyperCel Resins Mixed-mode Chromatography for Protein Separation
en 825.8 kB


  • Application
    HTPD resin screening

Compliance Information

  • Quality Standards


  • Bead Size
    80 - 100 µm
  • Bed Height
    10 mm
  • Dimensions
    5 x 10 mm

General Specifications

  • Flow Rate
    16-1000 cm/hr
  • Resin Format
    Pre-packed column

Materials of Construction

  • Base Matrix
    Rigid cellulose beads
  • Materials of Construction

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)
  • pH Stability
    1 to 14 (short term); 2 to 12 (long term)

Physicochemical Information

  • Bed Volume
    200 µL per column
  • Ligand
  • Reference Protein
  • Typical Dynamic Binding Capacity 10% per mL
    40-60 mg/mL BSA

Product Information

  • Pack Size
    1 row of 8
  • Pack Volume
    200 µL
  • Primary Product Type
    Pre-packed column
  • Resin Delivery Condition
    1M NaCl with 20% EtOH
  • Resin Type
    Mixed-mode anion exchanger
  • What you receive
    1 row of 8 columns

Storage Conditions

  • Storage Conditions
    20% EtOH/1M NaCl