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PRC Column 5x50 HEA HyperCel

HEA HyperCel is a mixed-mode resin with a less hydrophobic hexylamine ligand allowing protein binding at a moderate salt concentration under neutral pH. Pre-packed columns simplify resin screening and selection, and are ideal for process step optimization. The RoboColumn format is especially suited to high throughput process development (HTPD).

Item no.:  PRC05X050HEAHCEL

   

HEA HyperCel resin is an industry-scalable chromatography resin designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a “mixed-mode” mechanism, with a hexylamine ligand, its behavior is based on a combination of electrostatic and hydrophobic interactions of the proteins with the ligands. HEA HyperCel resin provides unique selectivities, different from those given by ion exchange or conventional HIC (hydrophobic interaction chromatography).

 

Features and Benefits

- High mechanical and chemical stability
- Suitable for large-scale chromatography in a biopharmaceutical environment
- Cross-linked cellulose base matrix


Applications

- Protein purification at low ionic strength by direct hydrophobic capture
- Separation of challenging mixtures, e.g. protein isoforms or proteins with very close pIs
- Orthogonal separation mechanism to ion exchange or HIC

 

Available Formats for HEA HyperCel

Pack SizeOrder Code
25 mL20250-026
100 mL20250-033
1 L20250-041
5 L20250-042
10 L20250-056

 

As pre-packed columns:

Pack SizeOrder Code
1 mL PRC Prepacked Column, 5 mm ID x 50 mmPRC05X050HEAHCEL
5 mL PRC Prepacked Column, 8 mm ID x 100 mmPRC08X100HEAHCEL
RoboColumn HEA HyperCel 200 μL, row of 8SR2HEA
RoboColumn HEA HyperCel 600 μL, row of 8SR6HEA

 

Datasheet

HEA and PPA Hypercel Resins
en 832.1 kB

Applications

  • Application
    Resin screening and process optimization

Compliance Information

  • Quality Standards
    CoA

Dimensions

  • Bead Size
    80 - 100 µm
  • Bed Height
    50 mm
  • Dimensions
    5 x 50 mm

General Specifications

  • Flow Rate
    100-300cm/h
  • Resin Format
    Pre-packed column

Materials of Construction

  • Base Matrix
    Rigid cellulose beads
  • Materials of Construction
    Polypropylene

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)
  • pH Stability
    1 to 14 (short term); 2 to 12 (long term)

Physicochemical Information

  • Bed Volume
    1 mL
  • Ligand
    hexylamine
  • Reference Protein
    BSA
  • Typical Dynamic Binding Capacity 10% per mL
    40-60 mg/mL BSA

Product Information

  • Pack Size
    1 column per pack
  • Pack Volume
    1 mL
  • Primary Product Type
    Pre-packed column
  • Resin Delivery Condition
    24% iPrOH/100mM sodium phosphate, pH 4.3
  • Resin Type
    Mixed-mode anion exchanger
  • What you receive
    1 column, instructions

Storage Conditions

  • Storage Conditions
    20% EtOH/1 M NaCl