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PPA HyperCel, RoboColumn 600 µL, Mixed-Mode Chromatography Resin

PPA HyperCel is a mixed-mode resin with an aromatic ligand exhibiting stronger hydrophobicity and providing a different selectivity in protein purification. Pre-packed columns simplify resin screening and selection, and are ideal for process step optimization. The RoboColumn format is especially suited to high throughput process development (HTPD).

Item no.:  SR6PPA

   

PPA HyperCel resin is an industry-scalable chromatography resin designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a “mixed-mode” mechanism, with a phenlypropylamine ligand, its behavior is based on a combination of electrostatic and hydrophobic interactions of the proteins with the ligands. PPA HyperCel resin provides unique selectivities, different from those given by ion exchange or conventional HIC (hydrophobic interaction chromatography).

 

Features and Benefits

- High mechanical and chemical stability
- Suitable for large-scale chromatography in a biopharmaceutical environment
- Cross-linked cellulose base matrix


Applications

- Protein purification at low ionic strength by direct hydrophobic capture
- Separation of challenging mixtures, e.g. protein isoforms or proteins with very close pIs
- Orthogonal separation mechanism to ion exchange or HIC

 

Available Formats for PPA HyperCel

Pack SizeOrder Code
25 mL20260-025
100 mL20260-030
1 L20260-040
5 L20260-045
10 L20260-052

 

As pre-packed columns:

Pack SizeOrder Code
1 mL PRC Prepacked Column, 5 mm ID x 50 mmPRC05X050PPAHCEL
5 mL PRC Prepacked Column, 8 mm ID x 100 mmPRC08X100PPAHCEL
RoboColumn PPA HyperCel 200 μL, row of 8SR2PPA
RoboColumn PPA HyperCel 600 μL, row of 8SR6PPA

 

Datasheet

HEA and PPA Hypercel Resins
en 832.1 kB

Applications

  • Application
    HTPD resin screening

Compliance Information

  • Quality Standards
    CoC

Dimensions

  • Bead Size
    80 - 100 µm
  • Bed Height
    30 mm
  • Dimensions
    5 x 30 mm

General Specifications

  • Flow Rate
    16-1000 cm/hr
  • Resin Format
    Pre-packed column

Materials of Construction

  • Base Matrix
    Rigid cellulose beads
  • Materials of Construction
    Polypropylene

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)
  • pH Stability
    1 to 14 (short term); 2 to 12 (long term)

Physicochemical Information

  • Bed Volume
    600 µL per column
  • Ligand
    phenylpropylamine
  • Reference Protein
    BSA
  • Typical Dynamic Binding Capacity 10% per mL
    40-60 mg/mL BSA

Product Information

  • Pack Size
    1 row of 8
  • Pack Volume
    600 µL
  • Primary Product Type
    Pre-packed column
  • Resin Delivery Condition
    1M NaCl with 20% EtOH
  • Resin Type
    Mixed-mode anion exchanger
  • What you receive
    1 row of 8 columns

Storage Conditions

  • Storage Conditions
    20% EtOH/1M NaCl