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PPA HyperCel, PRC Column 8 x 100, 5 mL Mixed-Mode Chromatography Resin

PPA HyperCel is a mixed-mode resin with an aromatic ligand exhibiting stronger hydrophobicity and providing a different selectivity in protein purification. Pre-packed columns simplify resin screening and selection, and are ideal for process step optimization. The RoboColumn format is especially suited to high throughput process development (HTPD).

Item no.:  PRC08X100PPAHCEL


PPA HyperCel resin is an industry-scalable chromatography resin designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a “mixed-mode” mechanism, with a phenlypropylamine ligand, its behavior is based on a combination of electrostatic and hydrophobic interactions of the proteins with the ligands. PPA HyperCel resin provides unique selectivities, different from those given by ion exchange or conventional HIC (hydrophobic interaction chromatography).


Features and Benefits

- High mechanical and chemical stability
- Suitable for large-scale chromatography in a biopharmaceutical environment
- Cross-linked cellulose base matrix


- Protein purification at low ionic strength by direct hydrophobic capture
- Separation of challenging mixtures, e.g. protein isoforms or proteins with very close pIs
- Orthogonal separation mechanism to ion exchange or HIC


Available Formats for PPA HyperCel

Pack SizeOrder Code
25 mL20260-025
100 mL20260-030
1 L20260-040
5 L20260-045
10 L20260-052


As pre-packed columns:

Pack SizeOrder Code
1 mL PRC Prepacked Column, 5 mm ID x 50 mmPRC05X050PPAHCEL
5 mL PRC Prepacked Column, 8 mm ID x 100 mmPRC08X100PPAHCEL
RoboColumn PPA HyperCel 200 μL, row of 8SR2PPA
RoboColumn PPA HyperCel 600 μL, row of 8SR6PPA



HEA and PPA Hypercel Resins
en 832.1 kB


  • Application
    Resin screening and process optimization

Compliance Information

  • Quality Standards


  • Bead Size
    80 - 100 µm
  • Bed Height
    100 mm
  • Dimensions
    8 x 100 mm

General Specifications

  • Flow Rate
  • Resin Format
    Pre-packed column

Materials of Construction

  • Base Matrix
    Rigid cellulose beads
  • Materials of Construction

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)
  • pH Stability
    1 to 14 (short term); 2 to 12 (long term)

Physicochemical Information

  • Bed Volume
    5 mL
  • Ligand
  • Reference Protein
  • Typical Dynamic Binding Capacity 10% per mL
    40-60 mg/mL BSA

Product Information

  • Pack Size
    1 column per pack
  • Pack Volume
    5 mL
  • Primary Product Type
    Pre-packed column
  • Resin Delivery Condition
    24% iPrOH/100mM sodium phosphate, pH 4.3
  • Resin Type
    Mixed-mode anion exchanger
  • What you receive
    1 column, instructions

Storage Conditions

  • Storage Conditions
    20% EtOH/1M NaCl