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MEP HyperCel, PRC Column 8 x 100, 5 mL Mixed-Mode Chromatography Resin

MEP HyperCel provides a unique separation mechanism and selectivity for protein separations. The MEP ligand facilitates the binding of immunoglobulins as well as other target proteins at neutral pH under moderate salt concentrations. Pre-packed columns simplify resin screening and selection, and are ideal for process step optimization. The RoboColumn format is especially suited to high throughput process development (HTPD).

Item no.:  PRC08X100MEPHCEL

   

MEP HyperCel is a flexible mixed-mode chromatography resin designed for capture and purification of antibodies and various recombinant proteins from laboratory to manufacturing scale.

MEP HyperCel resin offers a no salt/low salt alternative to conventional HIC and can simplify processes and improve economics by removing the need for salt addition and following UF/DF steps. The MEP ligand is immunoglobulin-selective. Thus, MEP Hypercel can be effectively used for IgG capture and for enhanced DNA, HCP and aggregate removal. Elution is under mild conditions.

 

Features and Benefits

- Unique separation mechanism and differentiated selectivity
- Direct protein capture from a variety of feedstocks
- No salt addition required for binding means a simpler process, lower costs and reduced waste
- Stable toward harsh cleaning with NaOH


Applications

- A no-salt/low-salt alternative to Hydrophobic Interaction Chromatography (HIC)
- Monoclonal and polyclonal IgG capture and intermediate purification (aggregate, DNA and HCP removal)
- Direct capture of recombinant proteins or enzymes from feedstock without need for pH or conductivity adjustments

Available formats for MEP HyperCel

Pack SizeOrder Code
25 mL12035-010
100 mL12035-028
1 L12035-036
5 L12035-040
10 L12035-044

 

 As pre-packed columns:

Pack SizeOrder Code
PRC Column 5x50 MEP HyperCel, 1 mLPRC05X050MEPHCEL
PRC Column 8x100 MEP HyperCel, 5 mLPRC08X100MEPHCEL
RoboColumn MEP HyperCel 200µLSR2MEP
RoboColumn MEP HyperCel 600µLSR6MEP

 

Datasheet

MEP HyperCel Mixed-Mode Chromatography Resin
en 724.2 kB

Applications

  • Application
    Resin screening and process optimization

Compliance Information

  • Quality Standards
    CoA

Dimensions

  • Bead Size
    80 - 100 µm
  • Bed Height
    100 mm
  • Dimensions
    8 x 100 mm

General Specifications

  • Flow Rate
    200-600cm/h
  • Resin Format
    Pre-packed column

Materials of Construction

  • Base Matrix
    Rigid cellulose beads
  • Materials of Construction
    Polypropylene

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)
  • pH Stability
    2 to 14 (short term); 2 to 12 (long term)

Physicochemical Information

  • Bed Volume
    5 mL
  • Ligand
    4-mercaptoethylpyridine
  • Reference Protein
    hIgG
  • Typical Dynamic Binding Capacity 10% per mL
    ≥ 20 mg/mL hIgG at 6' residence time

Product Information

  • Pack Size
    1 column per pack
  • Pack Volume
    5 mL
  • Primary Product Type
    Pre-packed column
  • Resin Delivery Condition
    1M NaCl with 20% EtOH
  • Resin Type
    Mixed-mode anion exchanger
  • What you receive
    1 column, instructions

Storage Conditions

  • Storage Conditions
    20% EtOH/1M NaCl