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HEA HyperCel - 5 L

HEA HyperCel is a mixed-mode resin with a less hydrophobic hexylamine ligand allowing protein binding at a moderate salt concentration under neutral pH.

Item no.:  20250-042

   

HEA HyperCel resin is an industry-scalable chromatography resin designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a “mixed-mode” mechanism, with a hexylamine ligand, its behavior is based on a combination of electrostatic and hydrophobic interactions of the proteins with the ligands. HEA HyperCel resin provides unique selectivities, different from those given by ion exchange or conventional HIC (hydrophobic interaction chromatography).

 

Features and Benefits

- High mechanical and chemical stability
- Suitable for large-scale chromatography in a biopharmaceutical environment
- Cross-linked cellulose base matrix


Applications

- Protein purification at low ionic strength by direct hydrophobic capture
- Separation of challenging mixtures, e.g. protein isoforms or proteins with very close pIs
- Orthogonal separation mechanism to ion exchange or HIC

 

Available Formats for HEA HyperCel

Pack SizeOrder Code
25 mL20250-026
100 mL20250-033
1 L20250-041
5 L20250-042
10 L20250-056

 

As pre-packed columns:

Pack SizeOrder Code
1 mL PRC Prepacked Column, 5 mm ID x 50 mmPRC05X050HEAHCEL
5 mL PRC Prepacked Column, 8 mm ID x 100 mmPRC08X100HEAHCEL
RoboColumn HEA HyperCel 200 μL, row of 8SR2HEA
RoboColumn HEA HyperCel 600 μL, row of 8SR6HEA

 

Datasheet

HEA and PPA HyperCel Resins Mixed-mode Chromatography for Protein Separation
en 825.8 kB

Applications

  • Application
    Preparative protein purification

Compliance Information

  • Quality Standards
    CoA, RSF

Dimensions

  • Bead Size
    80 - 100 µm

General Specifications

  • Flow Rate
    100-600cm/h
  • Resin Format
    Bulk resin

Materials of Construction

  • Base Matrix
    Rigid cellulose beads

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)
  • pH Stability
    1 to 14 (short term); 2 to 12 (long term)

Physicochemical Information

  • Ligand
    hexylamine
  • Reference Protein
    BSA
  • Typical Dynamic Binding Capacity 10% per mL
    40-60 mg/mL BSA

Product Information

  • Pack Size
    1
  • Pack Volume
    5 L
  • Primary Product Type
    Resins
  • Resin Delivery Condition
    1M NaCl with 20% EtOH
  • Resin Type
    Mixed-mode anion exchanger
  • What you receive
    1 bottle resin, instructions

Storage Conditions

  • Storage Conditions
    20% EtOH/1 M NaCl