Sartobind STIC® PA 200 mL, 4 mm bed height
Sartobind STIC® PA (primary amine) anion exchange membrane overcomes reduced binding capacity for host cell protein removal and can be used in high-salt conditions.
Item no.: 96STPA42D1GSS
Availability (ex works): On Request
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Sartobind STIC® PA removes contaminants at high salt. Salt-tolerant interaction chromatography (STIC) with PA ligand is based on anion-exchange chromatography (AEX) principles. AEX is the established method for removing process-derived contaminants such as host-cell proteins (HCP), DNA, endotoxins, and adventitious and endogenous viruses. It is performed during the downstream processing of monoclonal antibodies (mAbs) in flow-through mode. The process is driven by throughput and not by mass, and thus membrane chromatography (MC) is advantageous over columns for contaminant removal, as membranes run up to two orders of magnitude higher flow rates. Membranes are easy to operate and can be disposed of after one use, just like filters, to save on validation cost.
Current mAb downstream processes consist of at least one initial capture and one AEX chromatography step. The limitation of AEX steps is often that the Q chemistry requires low-salt conditions. In processes with cation exchange (CEX) as the second chromatography step, the CEX pool needs to be diluted to conductivities of 4-7 mS/cm to reestablish binding conditions for the following AEX step. The Sartobind STIC PA membrane is composed of cross-linked, regenerated macro-porous cellulose. The primary amine ligand is attached to the matrix at approximately six-fold higher ligand density than that of Q membrane. Both the free amine and the high ligand density were crucial for developing its high salt tolerance.
The Sartobind STIC® adsorber speeds up the production process by high flow rate and opens new opportunities for the purification of therapeutic proteins and vaccines.
- No dilution of CEX pools necessary
- Host cell proteins up to 10kg/l (product load/liter membrane)
- Viruses >4 log
- DNA at up to 1.5 M NaCl
- Endotoxins >4 log
Easy to use:
- Plug and play devices from 0.08 ml to 1.6 L
- Can be used like a filter
- Flow rate up to 30 bed volumes/min
- Disposable after one use
Reference ProteinBovine serum albumin
BiosafetyAll materials of this filter element meet the requirements of the current USP Biological Reactivity tests for plastics Class VI (Systemic Injection, Intracutaneous and Implantation tests).
ConnectorsSanitary 1 - 1 ½"
Bed Height4 mm
Dimensions (W×D×H)10 × 35 cm
Membrane Area0.73 m²
Materials of Construction
Housing MaterialPolypropylene (PP)
Materials of ConstructionPolypropylene, polyester
Membrane MaterialStabilised reinforced cellulose
Maximum Operating Pressure0.4 MPa
Maximum Venting Pressure0.5 bar, 0.05 MPa, 7 psi
Thermal StabilitySartobind capsules with ion exchange membranes can be autoclaved at 121 °C for 30 min. We recommend one autoclaving cycle only.
Chemical CompatibilityStable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
Ligand Density18 – 22 µeq/cm²
Pore Size Final-filter3 – 5 µm
Typical Dynamic Binding Capacity 10% per unit10 g
Membrane TypeSalt tolerant interaction membrane (anion exchanger)
Primary Product TypeMembranes
What you receive
1 operating instruction
- 1 certificate
- 1 capsule
Flow Rate4 L/min
LigandPrimary amine (PA)
Membrane Adsorber FormatCapsules
pH StabilitypH 2 – 14 (short term)
Bed Volume200 mL
Void Volume540 mL