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Sartobind® Q Cassette 1.6 L, 8 mm bed height

Sartobind® Q strong ion exchangers are perfect tools for the polishing of monoclonal antibodies for the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins. As size exclusion effects are neglectible, they are suited for purification of viruses such as adeno or lentiviruses displaying one order of magnitude higher dynamic binding capacity.

The cassette design eliminates the 5 L size limitation for membrane adsorbers and offers simple pod-like modular system up to 100 L membrane volume.

Item no.:  98IEXQ42E-P

Availability (ex works):  On Request

   

Sartobind® Q membrane adsorbers display a macro-porous structure with pore size of > 3 µm. Quaternary ammonium ligands are bound covalently to the complete internal surface of the membrane, resulting in separation media of high binding capacity combined with exceptional high flow rates.

Applications virus and VLP capture:

As size exclusion effects are negligible, they are suited for purification of viruses and display one order of magnitude higher dynamic binding capacity compared to resin. Viruses such as adeno- or lentiviruses are captured at high flow rates. The material allows also >100 cycles of re-use without loss of binding capacity.

Benefits capture:

  • High speed purification at 5 - 30 bed volumes per minute
  • No size exclusion effects for large proteins an viruses / VLPs
  • >10fold higher binding capacities compared to conventional resins

Applications flow-through polishing:

Sartobind Q is the perfect tool for the flow-through polishing of valuable proteins such as monoclonal antibodies. The bed volumes can be kept sufficiently small during the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins from such pharmaceutical proteins.

Benefits flow-through polishing:

  • Virus clearance > 6 log
  • DNA below detection limit
  • Host cell protein removal > 99 %
  • Endotoxin removal > 5 log
  • Save 80 % of column costs

Easy to use:

  • Plug and play devices from 0.08 mL to 5 L
  • Can be used like a filter
  • Flow rate up to 30 bed volumes/min
  • Re-usable or disposable

Application Note

Sartobind ® Capsules for Capture and Polishing
en 811.2 kB

Brochure

Sartobind® Membrane Chromatography
en 3.7 MB

Datasheet

Sartobind® Cassettes, 4 and 8 mm bed height
en 2.5 MB

Flyer

Sartobind® selection guide
en 1.0 MB

User Manual

Void Volume Optimized Capsules and Cassettes With 4 | 8 mm Bed Height
en 2.1 MB

Applications

  • Application
    • Capture
    • Polishing

Compliance Information

  • Biosafety
    All materials of this filter element meet the requirements of the current USP Biological Reactivity tests for plastics Class VI (Systemic Injection, Intracutaneous and Implantation tests).

Connections (Physical)

  • Connectors
    Sanitary 1 – 1 ½" via Manifold

Dimensions

  • Bed Height
    8 mm
  • Dimensions (W×D×H)
    634 × 49 × 387 mm
  • Membrane Area
    5.8 m²

Features

  • Reusable
    Yes

Materials of Construction

  • Housing Material
    Acrylonitrile butadiene styrene (ABS)
  • Materials of Construction
    Polyester
  • Membrane Material
    Stabilised reinforced cellulose

Operating Conditions

  • Maximum Operating Pressure
    0.2 MPa
  • Maximum Venting Pressure
    0.5 bar, 0.05 MPa, 7 psi
  • Recommended Flow Rate
    8 L/min
  • pH Stability
    pH 2 – 14 (short term), pH 2 – 12 (long term)

Physicochemical Information

  • Bed Volume
    1.6 L
  • Chemical Compatibility
    Stable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
  • Ligand
    Quaternary ammonium
  • Ligand Density
    2 – 5 µeq/cm²
  • Pore Size
    3 – 5 µm
  • Reference Protein
    Bovine serum albumin
  • Thermal Stability
    Sartobind cassettes with ion exchange membranes cannot be autoclaved, but they can be gamma irradiated.
  • Typical Dynamic Binding Capacity 10% per unit
    46 g
  • Void Volume
    2.9 L

Product Information

  • Membrane Adsorber Format
    Cassettes
  • Membrane Type
    Strong anion exchange membrane
  • Pack Size
    1
  • Primary Product Type
    Membranes
  • Sterilization Method
    Autoclavable
  • Type
    Membrane adsorber
  • What you receive
    • 1 cassette
    • 1 operating instruction
    • 1 certificate
Pilot Filter Holder — For Sartoclear® Depth Filters and Sartobind® Membrane Adsorbers

Filter Holders are hydraulic clamping systems especially designed for the use of modular Sartoclear® Depth Filter cassettes and Sartobind® membrane chromatography cassettes. The holders are available in three standard formats for pilot scale and commercial manufacturing.

Process Filter Holder — For Sartoclear® Depth Filters and Sartobind® Membrane Adsorbers

Filter Holders are hydraulic clamping systems especially designed for the use of modular Sartoclear® Depth Filter cassettes and Sartobind® membrane chromatography cassettes. The holders are available in three standard formats for pilot scale and commercial manufacturing.

Double Process Filter Holder — For Sartoclear® Depth Filters and Sartobind® Membrane Adsorbers

Filter Holders are hydraulic clamping systems especially designed for the use of modular Sartoclear® Depth Filter cassettes and Sartobind® membrane chromatography cassettes. The holders are available in three standard formats for pilot scale and commercial manufacturing.

Manifold Set for Sartoclear  Depth Filters and Sartobind® Membrane Adsorbers

The manifold set contains the inlet and outlet adapter plates for Sartoclear® Depth Filters as well as Sartobind® membrane adsorbers cassettes.