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Sartobind® Phenyl 96-Well Plates

Sartobind® Phenyl accomplishes polishing and capturing ten times faster than do conventional HIC resins. The membrane shows comparable, high binding capacity, without size exclusion effects, for proteins in a range of 20 mg mAb/mL membrane, as well as excellent resolution and impurity reduction.

Item no.:  99HICP42GC-----V

Availability:  On Request

Hydrophobic interaction chromatography (HIC) separates and purifies biomolecules based on differences in their hydrophobicity. The phenyl membrane adsorber follows the same rules known from the conventional hydrophobic interaction chromatography. Due to the large pore size, membrane adsorbers show excellent flow properties. There is almost no diffusion limitation of mass transport compared with conventional bead chromatography. Buffers with high concentrations of salt promote the adsorption of proteins on the hydrophobic membrane matrix. Proteins are eluted by decreasing the salt concentration in the elution buffer.

The salt dependence of the membrane is comparable to that of columns with ammonium sulfate, sodium chloride and sodium citrate.

Applications:

HIC is a powerful method for differentiating target protein and hydrophobic contaminants. Large proteins bind more strongly than do small ones and aggregates even considerably better than monomers due to multipoint attachment.

Buffer with a high ionic strength, usually ammonium sulfate, is initially added to the protein solution. Salt ions in this solution reduce the solvation of solutes in the sample. As solvation decreases, hydrophobic regions that become exposed are adsorbed by the hydrophobic membrane matrix. In the HIC membrane, flexible cellulose is used as a backbone for the phenyl ligand. The membrane can be regenerated by using either water or alcohol.

The HIC membrane is used for the removal of:

  • Aggregates
  • Host cell proteins
  • Viruses
  • Endotoxins
  • Lipids, dyes, detergents and anti foam agents
  • Leached chromatography ligands

The Phenyl membrane can be used also for binding large proteins such as:

  • Monoclonal antibodies
  • Conjugated vaccines, viruses and phages
  • Oligonucleotides

Benefits:

HIC membranes offer the following advantages over conventional HIC columns:

  • Excellent reduction of aggregates
  • 85 % buffer savings
  • 75 % of time saved

Easy to use:

  • Plug and play devices from 0.08 ml to 5 l
  • Can be used like a filter
  • Flow rate up to 5 bed volumes/min
  • Re-usable >100 cycles or disposable

Application Note

Phenyl Membrane Adsorber for Bioprocessing
en 1.8 MB

Brochure

Sartobind® Membrane Adsorbers — Chromatography as easy as filtration
en 3.7 MB

Datasheet

Sartobind® 96-well plates — High Throughput Screening with IEX and HIC Membrane Chromatography
en 843.2 kB

Flyer

Sartobind® selection guide
en 1.0 MB

User Manual

Sartobind® 96-well plates — IEX (Q, S, STIC PA) and HIC (Phenyl) in 8-strips — For High Throughput Screening Based on Macroporous Membranes, 0.8 mm Bed Height
en 300.6 kB

Product properties

  • Connectors
    N/A
  • Max. operating pressure
    35 kPa
  • Pore size final-filter
    3 – 5 µm
  • Bed volume
    19 µL/well
  • Bed height
    0.8 mm
  • Membrane area
    0.7 cm²
  • Primary Product Type
    Membranes
  • Type
    Membrane adsorber (96-well plate)
  • Design
    Conventional filter design
  • Filter Format
    96-Well Plates
  • Membrane Material
    Stabilised reinforced cellulose
  • Pack Size
    2

Technical attributes

  • Dimensions (WxDxH)
    128 × 85.5 × 25 mm
  • Maximum Pressure
    35 kPa
  • Ligand density
    3 µeq/cm²
  • Typical dynamic binding capacity 10% per unit
    0.28 mg/well
  • Chemical Compatibility
    Stable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
  • Reference Protein
    Gamma globulin from bovine blood
  • Application
    Screening
  • What you receive
    • 2 plates
    • 4 deep well collection plate 2 ml
    • 1 operating instruction
  • Materials of Construction
    Polyester
  • Housing Material
    Polypropylene (PP)
  • Ligand
    Phenyl
  • Material Holding Frame
    Polystyrene
  • Material 8-strip
    Polypropylene (PP)
  • Membrane Type
    Hydrophobic interaction chromatography membrane (HIC)
  • pH Stability
    pH 2 – 14 (short term)
  • Reusable
    No