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RoboColumn HEA HyperCel 200 µL

HEA HyperCel is a mixed-mode resin with a less hydrophobic hexylamine ligand allowing protein binding at a moderate salt concentration under neutral pH. Pre-packed columns simplify resin screening and selection, and are ideal for process step optimization. The RoboColumn format is especially suited to high throughput process development (HTPD).

Item no.:  SR2HEA

Availability:  On Request

   

HEA HyperCel resin is an industry-scalable chromatography resin designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a “mixed-mode” mechanism, with a hexylamine ligand, its behavior is based on a combination of electrostatic and hydrophobic interactions of the proteins with the ligands. HEA HyperCel resin provides unique selectivities, different from those given by ion exchange or conventional HIC (hydrophobic interaction chromatography).

Features and Benefits

- High mechanical and chemical stability
- Suitable for large-scale chromatography in a biopharmaceutical environment
- Cross-linked cellulose base matrix


Applications

- Protein purification at low ionic strength by direct hydrophobic capture
- Separation of challenging mixtures, e.g. protein isoforms or proteins with very close pIs
- Orthogonal separation mechanism to ion exchange or HIC

Datasheet

HEA and PPA HyperCel Resins Mixed-mode Chromatography for Protein Separation
en 825.8 kB

Compliance Information

  • Quality Standards
    CoC

Dimensions

  • Bead Size
    80 - 100 µm
  • Bed Height
    10 mm
  • Dimensions
    5 x 10 mm

General Specifications

  • Flow Rate
    16-1000 cm/hr
  • Resin Format
    Pre-packed column

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)
  • pH Stability
    1 to 14 (short term), 2 to 12 (long term)

Storage Conditions

  • Storage Conditions
    20% EtOH/1M NaCl

eShop Dynamic Attributes

  • Application
    HTPD resin screening
  • Base Matrix
    Rigid cellulose beads
  • Bed Volume
    200 µL per column
  • Ligand
    hexylamine
  • Materials of Construction
    Polypropylene
  • Pack Size
    1 row of 8
  • Pack Volume
    200 µL
  • Reference Protein
    BSA
  • Resin Delivery Condition
    1M NaCl with 20% EtOH
  • Resin Type
    Mixed-mode anion exchanger
  • Typical Dynamic Binding Capacity 10% per mL
    40-60 mg/mL BSA
  • What you receive
    1 row of 8 columns