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PRC Column 8x100 HEA HyperCel

HEA HyperCel is a mixed-mode resin with a less hydrophobic hexylamine ligand allowing protein binding at a moderate salt concentration under neutral pH. Pre-packed columns simplify resin screening and selection, and are ideal for process step optimization. The RoboColumn format is especially suited to high throughput process development (HTPD).

Item no.:  PRC08X100HEAHCEL

Availability:  On Request

   

HEA HyperCel resin is an industry-scalable chromatography resin designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a “mixed-mode” mechanism, with a hexylamine ligand, its behavior is based on a combination of electrostatic and hydrophobic interactions of the proteins with the ligands. HEA HyperCel resin provides unique selectivities, different from those given by ion exchange or conventional HIC (hydrophobic interaction chromatography).

Features and Benefits

- High mechanical and chemical stability
- Suitable for large-scale chromatography in a biopharmaceutical environment
- Cross-linked cellulose base matrix


Applications

- Protein purification at low ionic strength by direct hydrophobic capture
- Separation of challenging mixtures, e.g. protein isoforms or proteins with very close pIs
- Orthogonal separation mechanism to ion exchange or HIC

Datasheet

HEA and PPA HyperCel Resins Mixed-mode Chromatography for Protein Separation
en 825.8 kB

Dimensions

  • Bead Size
    80 - 100 µm
  • Bed Height
    100 mm

General Specifications

  • Flow Rate
    200-600cm/h
  • Resin Format
    Pre-packed column

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)
  • pH Stability
    1 to 14 (short term), 2 to 12 (long term)

Storage Conditions

  • Storage Conditions
    20% EtOH/1 M NaCl

eShop Dynamic Attributes

  • Base Matrix
    Rigid cellulose beads
  • Bed Volume
    5 mL
  • Ligand
    hexylamine
  • Materials of Construction
    Polypropylene
  • Pack Volume
    5 mL
  • Reference Protein
    BSA
  • Resin Delivery Condition
    24% iPrOH/100mM sodium phosphate, pH 4.3
  • Resin Type
    Mixed-mode anion exchanger
  • Typical Dynamic Binding Capacity 10% per mL
    40-60 mg/mL BSA
  • What you receive
    1 column, instructions