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HEA HyperCel - 100 mL

HEA HyperCel is a mixed-mode resin with a less hydrophobic hexylamine ligand allowing protein binding at a moderate salt concentration under neutral pH.

Item no.:  20250-033

   

HEA HyperCel resin is an industry-scalable chromatography resin designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a “mixed-mode” mechanism, with a hexylamine ligand, its behavior is based on a combination of electrostatic and hydrophobic interactions of the proteins with the ligands. HEA HyperCel resin provides unique selectivities, different from those given by ion exchange or conventional HIC (hydrophobic interaction chromatography).

Features and Benefits

- High mechanical and chemical stability
- Suitable for large-scale chromatography in a biopharmaceutical environment
- Cross-linked cellulose base matrix


Applications

- Protein purification at low ionic strength by direct hydrophobic capture
- Separation of challenging mixtures, e.g. protein isoforms or proteins with very close pIs
- Orthogonal separation mechanism to ion exchange or HIC

Datasheet

HEA and PPA HyperCel Resins Mixed-mode Chromatography for Protein Separation
en 825.8 kB

Dimensions

  • Bead Size
    80 - 100 µm

General Specifications

  • Flow Rate
    100-600cm/h
  • Resin Format
    Bulk resin

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)
  • pH Stability
    1 to 14 (short term), 2 to 12 (long term)

Storage Conditions

  • Storage Conditions
    20% EtOH/1 M NaCl

eShop Dynamic Attributes

  • Application
    Preparative protein purification
  • Base Matrix
    Rigid cellulose beads
  • Ligand
    hexylamine
  • Pack Size
    1
  • Pack Volume
    100 mL
  • Quality Standards
    • CoA
    • RSF
  • Reference Protein
    BSA
  • Resin Delivery Condition
    1M NaCl with 20% EtOH
  • Resin Type
    Mixed-mode anion exchanger
  • Typical Dynamic Binding Capacity 10% per mL
    40-60 mg/mL BSA
  • What you receive
    1 bottle resin, instructions