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HA Ultrogel® - 100 mL Hydroxyapatite Chromatography Resin

HA Ultrogel® is a composite material of cross-linked agarose and microcrystalline hydroxyapatite enclosed in the agarose matrix. The material shows mixed mode functionality based on cation exchange and metal affinity in the hydroxyapatite structure. A wide variety of applications include separation of proteins, peptides and nucleic acids, from laboratory to production scale.

Item no.:  24775-025

Availability:  On Request

   

HA Ultrogel® resin is a hydroxyapatite agarose composite resin for the separation of biomolecules from research and to manufacturing scale. Hydroxyapatite chromatography is considered to be a “pseudo-affinity” chromatography, or “mixed-mode” ion exchange. It has proven to be an effective purification mechanism in a variety of processes, providing biomolecule selectivity complementary to more traditional ion exchange or hydrophobic interaction techniques.

Features and Benefits

- Unique selectivity and separation mechanism, complementary to traditional IEX and HIC
- Protein purification in neutral, non-denaturating conditions
- High porosity, easy cleaning

Applications

- Purification of human serum proteins
- Purification of basic proteins, of phosphate-dependent proteins and enzymes, and of DNA-dependent enzymes
- Vaccine purification processes, e.g. Bordetella pertussis toxin
- Separation of plant proteins such as lectins, glycoproteins, glycosidases, phospholipidases, sulfohydrolases, sphingomyelinases, transferases, trehalases and kinases

Dimensions

  • Bead Size
    120 µm

General Specifications

  • Flow Rate
    < 100 cm/h
  • Resin Format
    Bulk resin

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)
  • pH Stability
    5 to 12 (working pH)

Storage Conditions

  • Storage Conditions
    20% EtOH/1 M NaCl

eShop Dynamic Attributes

  • Base Matrix
    Agarose
  • Ligand
    Hydroxyapatite
  • Pack Volume
    100 mL
  • Reference Protein
    Cytochrome C
  • Resin Delivery Condition
    1M NaCl with 20% EtOH
  • Resin Type
    Mixed Mode
  • Typical Dynamic Binding Capacity 10% per mL
    > 7 mg/mL Cytochrome C
  • What you receive
    1 bottle resin, instructions