CIMmultus® SO3 400 mL cGMP Compliant Monolithic Column (6 μm)

This high-performance cation exchange column is primarily intended for fast and efficient purification of biomolecules such as viruses, adeno-associated viruses (AAV), and virus-like particles (VLP) through their ionic interaction with the monolith.

Item No.: 
924.6157-6
Piece
1

*Custom/bulk order quotes are provided within 72 hours of request.

CIMmultus® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production. CIMmultus® can be used as single-use disposable or multi-use columns, and they are available in various volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.

CIMmultus® SO3 is a high-performance cation exchange column, primarily intended for fast and efficient purification of biomolecules, such as viruses, adeno-associated viruses (AAV), and virus-like particles (VLP) through their ionic interaction with the monolith.

SO3 (sulfonate) features:

  • Strong cation exchanger
  • Working range, pH 2–13
  • Binds molecules with predominantly positive charge, repels molecules a predominantly negative charge
  • Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
  • Main application: AAV capture step
  • High capacity for purification of virus and VLP particles, large proteins
  • Sanitizable with 1 M NaOH

Benefits:

  • Very fast processes by convective mass transport – resolution independent of flow
  • Ready to use columns
  • High-capacity for large molecules due to fully accessible void volume in the channels
  • Laminar flow minimizes shear stress resulting in high yields

Applications

  • Application
    Preparative Applications  

Connections (Physical)

  • Connectors
    TC 1" (25 mm), 8 mm ID bore  

Dimensions

  • Channel Size
    6 μm  
  • Column Tube Length
    75 mm  
  • Inner Diameter
    65 mm  
  • Outer Diameter
    105 mm  

Features

  • Reusable
    Yes  

General Specifications

  • Flow Rate
    40–3200 mL/min | 12–977 cm/h | 0.1–8 CV/min  

Materials of Construction

  • Materials of Construction
    Poly(glycidyl methacrylate -co- ethylene dimethacrylate)  

Operating Conditions

  • Maximum Operating Pressure
    1.0 MPa | 10 bar | 145 psi  
  • Recommended Operating Temperature
    4 °C (39 °F) to 40 °C (104 °F)  
  • pH Stability
    Working range 2–13, cleaning in place 1–14  

Physicochemical Information

  • Bed Volume
    400 mL  
  • Ligand
    SO3 (strong cation exchanger, sulfonate)  
  • Typical Dynamic Binding Capacity 10% per mL
    ≥ 5 mg Lysozyme/mL wet support  

Product Information

  • Brand
    CIMmultus®  
  • Package Type
    Individually Packed