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CIMmultus® DEAE 80 mL Monolithic Column (1.3 μm)

CIMmultus® is a unique and industry-proven line of pre-packed chromatographic monolithic columns featuring large flow-through channels, designed for working with large biomolecules such as viruses (AAV, adenovirus, lentivirus, influenza, bacteriophages), nucleic acids (mRNA, pDNA), virus-like particles and exosomes. CIMmultus® columns can be used as single-use disposable or multi-use columns.

Item no.:  611.5114-1.3

   

CIMmultus® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production.

Without the need for column packing, CIMmultus® Monoliths are ready for use out of the box.

This high-performance ion exchange column is primarily intended for fast and efficient purification of biomolecules such as large proteins, viruses and virus like particles through their ionic interaction with the monolith. The following information is being provided to ensure proper product care and optimal product performance.

DEAE (diethylamine) features:

  • Weak anion exchanger
  • Working range, pH 2–9
  • Binds molecules with predominantly negative charge, repels molecules a predominantly positive charge
  • Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
  • High capacity for plasmid DNA
  • Sanitizable with 1 M NaOH

 

  • Channel Size
    1.3 µm
  • Column Tube Length
    110 mm
  • Connectors
    TC 1 in. (25 mm), 3 mm ID bore
  • Inner Diameter
    15 mm
  • Outer Diameter
    34 mm
  • Package Type
    Individually Packed
  • Recommended Operating Temperature
    4 °C (39 °F) to 40 °C (104 °F)

 

  • Bed Volume
    80 mL
  • Chemical Compatibility
    All commonly used aqueous buffers, 1 M NaOH, 0.1 M HCl, 8 M urea, 6 M guanidine hydrochloride and 20 % ethanol solution. Avoid oxidising agents. Avoid prolonged exposure to concentrated acids (over 0.5 M) such as hydrochloric, sulphuric or acetic acids.
  • Flow Rate
    40–400 mL/min | 30–300 cm/h | 0.5–5 CV/min
  • Ligand
    DEAE (Weak anion exchanger, diethylamino)
  • Materials of Construction
    Poly(glycidyl methacrylate -co- ethylene dimethacrylate)
  • Maximum Operating Pressure
    2.0 MPa | 20 bar | 290 psi
  • Reusable
    Yes
  • Typical Dynamic Binding Capacity 10% per mL
    ≥ 20 mg BSA/ml wet support, BSA, 3.0 mg/mL, 20 mM Tris-HCI buffer, pH 7.4, flow rate 160 mL/min
  • pH Stability
    Working range 2–10, cleaning in place 1–14

 

  • Application
    Preparative Applications
  • GMP Compliance
    No