CIMmultus® DEAE 8 mL Monolithic Column (2 μm)

This high-performance ion exchange column is primarily intended for fast and efficient purification of biomolecules, especially for plasmid DNA, through their ionic interaction with the monolith.

Item No.: 
411.5114-2
Piece
1

*Custom/bulk order quotes are provided within 72 hours of request.

CIMmultus® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production. CIMmultus® can be used as single-use disposable or multi-use columns, and they are available in various volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.

CIMmultus® DEAE is a high-performance ion exchange column, primarily intended for fast and efficient purification of biomolecules, especially for plasmid DNA, through their ionic interaction with the monolith.

DEAE features:

  • Weak anion exchanger
  • Working range, pH 2–9
  • Binds molecules with a predominantly negative charge, repels molecules with a predominantly positive charge
  • Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode

Benefits:

  • Very fast processes by convective mass transport – resolution independent of flow
  • Ready to use columns
  • High-capacity for large molecules due to fully accessible void volume in the channels
  • Laminar flow minimizes shear stress resulting in high yields

Applications

  • Application
    Preparative Applications  

Connections (Physical)

  • Connectors
    10-32 UNF coned port, 0.0625" OD tubing connection  

Dimensions

  • Channel Size
    2 μm  
  • Column Tube Length
    56 mm  
  • Inner Diameter
    6.5 mm  
  • Outer Diameter
    15 mm  

Features

  • Reusable
    Yes  

General Specifications

  • Flow Rate
    0.8–100 mL/min | 2.4–335 cm/h | 0.1–12.5 CV/min  

Materials of Construction

  • Materials of Construction
    Poly(glycidyl methacrylate -co- ethylene dimethacrylate)  

Operating Conditions

  • Maximum Operating Pressure
    2.0 MPa | 20 bar | 290 psi  
  • Recommended Operating Temperature
    4 °C (39 °F) to 40 °C (104 °F)  
  • pH Stability
    Working range 2–10, cleaning in place 1–14  

Physicochemical Information

  • Bed Volume
    8 mL  
  • Ligand
    DEAE (weak anion exchanger, diethylamino)  
  • Typical Dynamic Binding Capacity 10% per mL
    ≥ 20 mg BSA/ml wet support, BSA, 3.0 mg/mL, 20 mM Tris-HCI buffer, pH 7.4, flow rate 16 mL/min  

Product Information

  • Brand
    CIMmultus®  
  • Package Type
    Individually Packed