CIMmultus® DEAE 8 mL Monolithic Column (2 μm)
CIMmultus® is a unique and industry-proven line of pre-packed chromatographic monolithic columns featuring large flow-through channels, designed for working with large biomolecules such as viruses (AAV, adenovirus, lentivirus, influenza, bacteriophages), nucleic acids (mRNA, pDNA), virus-like particles and exosomes. CIMmultus® columns can be used as single-use disposable or multi-use columns.
Item no.: 411.5114-2
CIMmultus® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production.
Without the need for column packing, CIMmultus® Monoliths are ready for use out of the box.
This high-performance ion exchange column is primarily intended for fast and efficient purification of biomolecules such as large proteins, viruses and virus like particles through their ionic interaction with the monolith. The following information is being provided to ensure proper product care and optimal product performance.
DEAE (diethylamine) features:
- Weak anion exchanger
- Working range, pH 2–9
- Binds molecules with predominantly negative charge, repels molecules a predominantly positive charge
- Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
- High capacity for plasmid DNA
- Sanitizable with 1 M NaOH
Connectors10-32 UNF coned port, 1/16’’ OD tubing connection
Channel Size2 µm
Column Tube Length56 mm
Inner Diameter6.5 mm
Outer Diameter15 mm
Flow Rate8–100 mL/min | 26–335 cm/h | 1–12 CV/min
Materials of Construction
Materials of ConstructionPoly(glycidyl methacrylate -co- ethylene dimethacrylate)
Maximum Operating Pressure2.0 MPa | 20 bar | 290 psi
Recommended Operating Temperature4 °C (39 °F) to 40 °C (104 °F)
pH StabilityWorking range 2–10, cleaning in place 1–14
Bed Volume8 mL
LigandDEAE (Weak anion exchanger, diethylamino)
Typical Dynamic Binding Capacity 10% per mL≥ 20 mg BSA/ml wet support, BSA, 3.0 mg/mL, 20 mM Tris-HCI buffer, pH 7.4, flow rate 16 mL/min
Package TypeIndividually Packed