CIMmultus® DEAE 8 mL Monolithic Column (1.3 μm)
This high-performance ion exchange column is primarily intended for fast and efficient purification of biomolecules, especially for plasmid DNA, through their ionic interaction with the monolith.
- Item No.:
- 411.5114-1.3
- Piece
- 1
*Custom/bulk order quotes are provided within 72 hours of request.
Choose your CIMmultus®
CIMmultus® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production. CIMmultus® can be used as single-use disposable or multi-use columns, and they are available in various volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.
CIMmultus® DEAE is a high-performance ion exchange column, primarily intended for fast and efficient purification of biomolecules, especially for plasmid DNA, through their ionic interaction with the monolith.
DEAE features:
- Weak anion exchanger
- Working range, pH 2–9
- Binds molecules with a predominantly negative charge, repels molecules with a predominantly positive charge
- Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
Benefits:
- Very fast processes by convective mass transport – resolution independent of flow
- Ready to use columns
- High-capacity for large molecules due to fully accessible void volume in the channels
- Laminar flow minimizes shear stress resulting in high yields
Applications
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ApplicationPreparative Applications
Connections (Physical)
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Connectors10-32 UNF coned port, 0.0625" OD tubing connection
Dimensions
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Channel Size1.3 μm
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Column Tube Length56 mm
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Inner Diameter6.5 mm
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Outer Diameter15 mm
Features
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ReusableYes
General Specifications
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Flow Rate0.8–100 mL/min | 2.4–335 cm/h | 0.1–12.5 CV/min
Materials of Construction
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Materials of ConstructionPoly(glycidyl methacrylate -co- ethylene dimethacrylate)
Operating Conditions
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Maximum Operating Pressure2.0 MPa | 20 bar | 290 psi
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Recommended Operating Temperature4 °C (39 °F) to 40 °C (104 °F)
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pH StabilityWorking range 2–10, cleaning in place 1–14
Physicochemical Information
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Bed Volume8 mL
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LigandDEAE (weak anion exchanger, diethylamino)
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Typical Dynamic Binding Capacity 10% per mL≥ 20 mg BSA/ml wet support, BSA, 3.0 mg/mL, 20 mM Tris-HCI buffer, pH 7.4, flow rate 16 mL/min
Product Information
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BrandCIMmultus®
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Package TypeIndividually Packed
CIM® Monolithic Well Plates ensure robust and reliable results for screening multiple chromatographic conditions simultaneously or high-throughput purification of large biomolecules such as viruses, nucleic acids, exosomes, bacteriophages, etc.
Available in:
- 24-well or 96-well plate design
- Varied monolith volumes
- Wide range of CIM® chemistries
CIMac™ Analytical Columns combine all elaborate characteristics of CIM monoliths at analytical scale and are your perfect tool for analysis and control of manufacturing processes, ideal for process analytical technology (PAT). Designed for fast and reproducible HPLC monitoring and quantitation of proteins, viruses, pDNA, and other large biomolecules, they allow you to achieve rapid, high-resolution and flow-independent separations in a matter of minutes.