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CIMac™ pDNA 0.3mL Analytical Column (1.4 µm)

CIMac™ Analytical Columns combine all elaborate characteristics of CIM monoliths at analytical scale and are your perfect tool for analysis and control of manufacturing processes, ideal for process analytical technology (PAT). Designed for fast and reproducible HPLC monitoring and quantitation of proteins, viruses, pDNA, and other large biomolecules, they allow you to achieve rapid, high-resolution and flow-independent separations in a matter of minutes.

Item no.:  150.8501-1.4

   

The CIMac™ pDNA 0.3 mL Analytical Columns are intended for fast and reproducible HPLC monitoring and quantitation of plasmid DNA and are able to separate different plasmid DNA isoforms. They can be used for the in-process and final control of pDNA samples at different stages of the purification process.

pDNA features:

  • Weak anion exchanger
  • Enables fast and reproducible monitoring and quantitation of pDNA purification, and high-resolution separation of DNA plasmid isoforms

 

  • Channel Size
    1.4 µm
  • Connectors
    10-32 UNF coned port, 1/16’’ OD tubing connection
  • Diameter
    5.2 mm
  • Recommended Operating Temperature
    4 °C (39 °F) to 40 °C (104 °F)

 

  • Bed Volume
    0.3 mL
  • Chemical Compatibility
    All commonly used aqueous buffers, 1 M NaOH, 0.1 M HCl, 8 M urea, 6 M guanidine hydrochloride and 20 % ethanol solution. Avoid oxidizing agents. Avoid prolonged use of concentrated acids (more than 0.5 M) like hydrochloric, sulphuric or acetic acid.
  • Flow Rate
    0.2 – 3 mL/min | 1 – 15 cm/min | 0.67 – 10 CV/min
  • Ligand
    DEAE (weak anion exchanger, diethylamino)
  • Materials of Construction
    Poly(glycidyl methacrylate -co- ethylene dimethacrylate)
  • Maximum Operating Pressure
    10 MPa | 100 bar | 1450 psi
  • Reusable
    Yes
  • pH Stability
    Working range 3–9, cleaning in place 1–14

 

  • Application
    Analytical Columns
  • Length
    15 mm