CIMac™ EDA 0.1 mL Analytical Column (2 µm)
CIMac™ Analytical Columns combine all elaborate characteristics of CIM monoliths at analytical scale and are your perfect tool for analysis and control of manufacturing processes, ideal for process analytical technology (PAT). Designed for fast and reproducible HPLC monitoring and quantitation of proteins, viruses, pDNA, and other large biomolecules, they allow you to achieve rapid, high-resolution and flow-independent separations in a matter of minutes.
Item no.: 110.5116-2
Availability: On Request
The CIMac™ EDA 0.1 mL Analytical Columns are weak anion-exchange monolithic columns, that bind biomolecules that are predominantly negatively charged over the pH working range of 3-9. In contrast to DEAE or QA monoliths it contains free primary amino groups, resulting in a promotion of not purely ion-exchange interaction.
EDA (ethylenediamine) features:
- Weak anion exchanger
- Working range, pH 3–9
- Binds molecules with predominantly negative charge, repels molecules a predominantly positive charge
- Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
- High capacity for plasmid DNA
- Sanitizable with 1 M NaOH
Connectors10-32 UNF coned port, 1/16’’ OD tubing connection
Channel Size2 µm
Flow Rate0.2 – 3 mL/min | 1 – 15 cm/min | 2 – 30 CV/min
Materials of Construction
Materials of ConstructionPoly(glycidyl methacrylate -co- ethylene dimethacrylate)
Maximum Operating Pressure16 MPa | 150 bar | 2175 psi
Recommended Operating Temperature5 °C (39 °F) to 40 °C (104 °F)
pH StabilityWorking range 2–10, cleaning in place 1–15
Bed Volume0.1 mL
Chemical CompatibilityAll commonly used aqueous buffers, 1 M NaOH, 0.1 M HCl, 8 M urea, 6 M guanidine hydrochloride and 20 % ethanol solution. Avoid oxidizing agents. Avoid prolonged use of concentrated acids (more than 0.5 M) like hydrochloric, sulphuric or acetic acid.
LigandEDA (weak anion exchanger, ethylene diamino)