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CIMac™ EDA 0.1 mL Analytical Column (2 µm)

CIMac™ Analytical Columns combine all elaborate characteristics of CIM monoliths at analytical scale and are your perfect tool for analysis and control of manufacturing processes, ideal for process analytical technology (PAT). Designed for fast and reproducible HPLC monitoring and quantitation of proteins, viruses, pDNA, and other large biomolecules, they allow you to achieve rapid, high-resolution and flow-independent separations in a matter of minutes.

Item no.:  110.5116-2

   

The CIMac™ EDA 0.1 mL Analytical Columns are weak anion-exchange monolithic columns, that bind biomolecules that are predominantly negatively charged over the pH working range of 3-9. In contrast to DEAE or QA monoliths it contains free primary amino groups, resulting in a promotion of not purely ion-exchange interaction.

EDA (ethylenediamine) features:

  • Weak anion exchanger
  • Working range, pH 3–9
  • Binds molecules with predominantly negative charge, repels molecules a predominantly positive charge
  • Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
  • High capacity for plasmid DNA
  • Sanitizable with 1 M NaOH

 

  • Channel Size
    2 µm
  • Connectors
    10-32 UNF coned port, 1/16’’ OD tubing connection
  • Diameter
    5.2 mm
  • Recommended Operating Temperature
    5 °C (39 °F) to 40 °C (104 °F)

 

  • Bed Volume
    0.1 mL
  • Chemical Compatibility
    All commonly used aqueous buffers, 1 M NaOH, 0.1 M HCl, 8 M urea, 6 M guanidine hydrochloride and 20 % ethanol solution. Avoid oxidizing agents. Avoid prolonged use of concentrated acids (more than 0.5 M) like hydrochloric, sulphuric or acetic acid.
  • Flow Rate
    0.2 – 3 mL/min | 1 – 15 cm/min | 2 – 30 CV/min
  • Ligand
    EDA (weak anion exchanger, ethylene diamino)
  • Materials of Construction
    Poly(glycidyl methacrylate -co- ethylene dimethacrylate)
  • Maximum Operating Pressure
    16 MPa | 150 bar | 2175 psi
  • Reusable
    Yes
  • pH Stability
    Working range 2–10, cleaning in place 1–15

 

  • Application
    Analytical Columns
  • Length
    4.95 mm