CIMmultus® Oligo dT18 8000 mL Monolithic Column (2 μm)
CIMmultus® is a unique and industry-proven line of pre-packed chromatographic monolithic columns featuring large flow-through channels, designed for working with large biomolecules such as viruses (AAV, adenovirus, lentivirus, influenza, bacteriophages), nucleic acids (mRNA, pDNA), virus-like particles and exosomes. CIMmultus® columns can be used as single-use disposable or multi-use columns.
Item no.: 1011.1219-2
Availability: On Request
CIMmultus® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production.
Without the need for column packing, CIMmultus® Monoliths are ready for use out of the box.
This high-performance ion exchange column is primarily intended for fast and efficient purification of biomolecules such as large proteins, viruses and virus like particles through their ionic interaction with the monolith. The following information is being provided to ensure proper product care and optimal product performance.
Oligo dT (deoxythymine) features:
- Oligo dT ligands covalently bound to a solid support
- Hybridises to the poly-adenylated tail which is present on most eukaryotic mRNAs, or synthetised onto the molecule during IVT
- Primarily intended for fast and efficient capture and purification of mRNA with a poly-adenylated tail, from various sources
- Can selectively remove impurities from the sample and is a convenient approach for initial purification of mRNA
ConnectorsTC 1 in. (25 mm), 8 mm ID bore
Channel Size2 µm
Column Tube Length410 mm
Inner Diameter255 mm
Outer Diameter300 mm
Flow Rate1000–8000 mL/min | 15–135 cm/h | 0.15–1 CV/min
Materials of Construction
Materials of ConstructionPoly(glycidyl methacrylate -co- ethylene dimethacrylate)
Maximum Operating Pressure0.7 MPa | 7 bar | 100 psi
Recommended Operating Temperature4 °C (39 °F) to 30 °C (86 °F)
pH StabilityLinkage stability: pH 2–13. Ligand stability not tested. Functionality retained after exposure to 0.1 M NaOH for a few hours.
Bed Volume8000 mL
LigandOligo dT18 coupled to CDI-activated matrix, C12 Linker
Ligand Density> 0.5 mg Oligo dT/mL wet support
Package TypeIndividually Packed