Skip to content Skip to navigation menu

NutriFreez® D10 Cryopreservation Medium

A chemically defined, animal component-free, protein-free cryopreservation solution

Item no.:  05-713-1C

Price:  $26.00

Availability (ex works):  For more information please log in


NutriFreez® D10 Cryopreservation Medium is a ready-to-use, animal component-free, serum-free, and protein-free cell freezing solution optimized for multiple cell lines.  When culturing cells in a serum-free environment, it is essential to also maintain serum-free conditions during cryopreservation.  

NutriFreez® D10 Cryopreservation Medium has been demonstrated to result in high rates of cell viability, proliferation, adherence (in relevant lines), and bioactivity/ expression following freezing and thawing.  Superior results were obtained both in comparison with serum-containing freezing media as well as competing serum-free products, making this an ideal product for both serum-containing and serum-free applications.

NutriFreez® D10 Cryopreservation Medium has been extensively tested on multiple cell types, including:

  • Human Mesenchymal Stem Cells (hMSC), from various sources (e.g. bone marrow (BM-MSC), adipose tissue (AT-MSC), umbilical cord tissue (UC-MSC) derived and dental pulp tissue (hMSC –DP)
  • Human Embryonic Stem Cells (hESC), Induced Pluripotent Stem Cells (iPSC)
  • Human Peripheral Blood Mononuclear Cells (PBMC’s)
  • Human Endothelial Cells (EC)
  • T cells, including Chimeric Antigen Receptors (CART) cells and Tumor-Infiltrating Lymphocytes (TIL’s)
  • Neuron Cells
  • Hybridomas
  • CHO cells
  • Vero Cells
  • Multiple mammalian cell lines: MRC-5, HEK-293, HepG2, HeLaBSC-1, BGM3T3, MA-10BHK-21 as well as other extremely sensitive cell types


NutriFreez® D10 Cryopreservation Medium Features

  • Cryoprotective formulation designed to minimize dehydration effects
  • Effective maintenance of cell viability, adhesion, and bioactivity
  • Ready-to-use solution, simple protocol
  • Suitable for use in cell banks and biopharmaceutical applications
  • Animal component-free, serum-free, protein-free solution
  • cGMP Manufactured
  • Drug Master File available

Results of a cryopreservation comparison performed at the Research and Development Center for Cell Therapy (Japan) on human pluripotent stem cells (hESC and hiPSC) show that NutriFreez® D10 Cryopreservation Medium* presents best recovery rate for hPSCs after thawing (Nishishita et al. Am J Stem Cells. 2015).

Furthermore, an evaluation of different cryopreservation agents for mesenchymal stem cells (MSC) performed at Ottawa Hospital Research Institute (Canda) shows that MSC cryopreserved in NutriFreez® D10 Cryopreservation Medium exhibit the best post-thaw viability (Salkhordeh et. al. Cytotherapy, May 2018).

* NutriFreez® brand replaces the brand CryoStem™


NutriFreez D10 Cryopreservation Medium
en 95.2 kB

Safety Datasheet

NutriFreez D10 Cryopreservation Medium MSDS (GB)
en 124.3 kB
NutriFreez D10 Cryopreservation Medium MSDS (US)
en 128.9 kB

Technical Note

NutriFreez™ D10 Cryopreservation Medium
en 4.0 MB

User Manual

NutriFreez D10 Cryopreservation Medium Instructions For Use
en 106.3 kB


  • Application Area
    Cryopreservation of cells

Compliance Information

  • Animal Origin (AO) / Non-Animal Origin (NAO)


  • Phenol Red Indicator
    With Phenol Red


  • Serum Free

Physicochemical Information

  • Adherence/Suspension
  • Formulation
    Animal-component Free


  • Delivery Condition

Storage Conditions

  • Storage Conditions
    Protected from light
  • Storage Temperature


  • Volume
    20 ML

Customers Also Purchased

NutriStem® hPSC XF Medium

NutriStem® hPSC XF Medium

Learn More
MSC NutriStem® XF Medium

MSC NutriStem® XF Medium

Learn More
EndoGo™ XF Medium

EndoGo™ XF Medium

Learn More