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NutriFreez® D10 Cryopreservation Medium

A chemically defined, animal component-free, protein-free cryopreservation solution

Item no.:  05-713-1B

Price:  $155.00

Availability (ex works):  For more information please log in

   

NutriFreez® D10 Cryopreservation Medium is a ready-to-use, animal component-free, serum-free, and protein-free cell freezing solution optimized for multiple cell lines.  When culturing cells in a serum-free environment, it is essential to also maintain serum-free conditions during cryopreservation.  

NutriFreez® D10 Cryopreservation Medium has been demonstrated to result in high rates of cell viability, proliferation, adherence (in relevant lines), and bioactivity/ expression following freezing and thawing.  Superior results were obtained both in comparison with serum-containing freezing media as well as competing serum-free products, making this an ideal product for both serum-containing and serum-free applications.

NutriFreez® D10 Cryopreservation Medium has been extensively tested on multiple cell types, including:

  • Human Mesenchymal Stem Cells (hMSC), from various sources (e.g. bone marrow (BM-MSC), adipose tissue (AT-MSC), umbilical cord tissue (UC-MSC) derived and dental pulp tissue (hMSC –DP)
  • Human Embryonic Stem Cells (hESC), Induced Pluripotent Stem Cells (iPSC)
  • Human Peripheral Blood Mononuclear Cells (PBMC’s)
  • Human Endothelial Cells (EC)
  • T cells, including Chimeric Antigen Receptors (CART) cells and Tumor-Infiltrating Lymphocytes (TIL’s)
  • Neuron Cells
  • Hybridomas
  • CHO cells
  • Vero Cells
  • Multiple mammalian cell lines: MRC-5, HEK-293, HepG2, HeLaBSC-1, BGM3T3, MA-10BHK-21 as well as other extremely sensitive cell types

 

NutriFreez® D10 Cryopreservation Medium Features

  • Cryoprotective formulation designed to minimize dehydration effects
  • Effective maintenance of cell viability, adhesion, and bioactivity
  • Ready-to-use solution, simple protocol
  • Suitable for use in cell banks and biopharmaceutical applications
  • Animal component-free, serum-free, protein-free solution
  • cGMP Manufactured
  • Drug Master File available

Results of a cryopreservation comparison performed at the Research and Development Center for Cell Therapy (Japan) on human pluripotent stem cells (hESC and hiPSC) show that NutriFreez® D10 Cryopreservation Medium* presents best recovery rate for hPSCs after thawing (Nishishita et al. Am J Stem Cells. 2015).

Furthermore, an evaluation of different cryopreservation agents for mesenchymal stem cells (MSC) performed at Ottawa Hospital Research Institute (Canda) shows that MSC cryopreserved in NutriFreez® D10 Cryopreservation Medium exhibit the best post-thaw viability (Salkhordeh et. al. Cytotherapy, May 2018).

* NutriFreez® brand replaces the brand CryoStem™

Brochure

NutriFreez D10 Cryopreservation Medium
en 95.2 kB

Safety Datasheet

NutriFreez D10 Cryopreservation Medium MSDS (GB)
en 124.3 kB
NutriFreez D10 Cryopreservation Medium MSDS (US)
en 128.9 kB

Technical Note

NutriFreez™ D10 Cryopreservation Medium
en 4.0 MB

User Manual

NutriFreez D10 Cryopreservation Medium Instructions For Use
en 106.3 kB

Applications

  • Application Area
    Cryopreservation

Compliance Information

  • Animal Origin (AO) / Non-Animal Origin (NAO)
    NAO

Features

  • Serum Free
    Yes

Physicochemical Information

  • Adherence/Suspension
    Adherent/Suspension
  • Formulation
    w/ Phenol red

Sterility

  • Delivery Condition
    Sterile

Storage Conditions

  • Storage Conditions
    Protected from light
  • Storage Temperature
    2-8°C

Volumes

  • Volume
    100 ML

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