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Sartobind® Q 96-Well Plates, 2 units

Sartobind® Q strong ion exchangers are perfect tools for the polishing of monoclonal antibodies for the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins. As size exclusion effects are neglectible, they are suited for purification of viruses such as adeno or lentiviruses displaying one order of magnitude higher dynamic binding capacity.

Item no.:  99IEXQ42GC-----V

Availability (ex works):  On Request

Sartobind® Q membrane adsorbers display a macro-porous structure with pore size of > 3 µm. Quaternary ammonium ligands are bound covalently to the complete internal surface of the membrane, resulting in separation media of high binding capacity combined with exceptional high flow rates.

Applications virus and VLP capture:

As size exclusion effects are negligible, they are suited for purification of viruses and display one order of magnitude higher dynamic binding capacity compared to resin. Viruses such as adeno- or lentiviruses are captured at high flow rates. The material allows also >100 cycles of re-use without loss of binding capacity.

Benefits capture:

  • High speed purification at 5 - 30 bed volumes per minute
  • No size exclusion effects for large proteins an viruses / VLPs
  • >10fold higher binding capacities compared to conventional resins

Applications flow-through polishing:

Sartobind Q is the perfect tool for the flow-through polishing of valuable proteins such as monoclonal antibodies. The bed volumes can be kept sufficiently small during the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins from such pharmaceutical proteins.

Benefits flow-through polishing:

  • Virus clearance > 6 log
  • DNA below detection limit
  • Host cell protein removal > 99 %
  • Endotoxin removal > 5 log
  • Save 80 % of column costs

Easy to use:

  • Plug and play devices from 0.08 mL to 5 L
  • Can be used like a filter
  • Flow rate up to 30 bed volumes/min
  • Re-usable or disposable

Application Note

High Throughput Screening using Membrane Chromatography — Membrane based 96-well plate for process development in biomanufacturing
en 1.0 MB

Brochure

Sartobind® Membrane Chromatography
en 3.7 MB
宣传手册:实验室过滤产品精简目录
zh 19.0 MB

Datasheet

Sartobind® 96-well plates — High Throughput Screening with IEX and HIC Membrane Chromatography
en 843.2 kB

Flyer

Sartobind® selection guide
en 1.0 MB

User Manual

Sartobind® 96-well plates — IEX (Q, S, STIC PA) and HIC (Phenyl) in 8-strips — For High Throughput Screening Based on Macroporous Membranes, 0.8 mm Bed Height
en 300.6 kB

Applications

  • Application
    Screening

Biological Information

  • Reference Protein
    Bovine serum albumin

Connections (Physical)

  • Connectors
    N/A

Dimensions

  • Bed Height
    0.8 mm
  • Dimensions (W×D×H)
    128 × 85.5 × 25 mm
  • Membrane Area
    0.7 cm²

Features

  • Reusable
    No

Materials of Construction

  • Housing Material
    Polypropylene (PP)
  • Material 8-strip
    Polypropylene (PP)
  • Material Holding Frame
    Polystyrene
  • Materials of Construction
    Polyester
  • Membrane Material
    Stabilised reinforced cellulose

Operating Conditions

  • Maximum Operating Pressure
    35 kPa

Physicochemical Information

  • Chemical Compatibility
    Stable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
  • Ligand Density
    2 – 5 µeq/cm²
  • Pore Size Final-filter
    3 – 5 µm
  • Typical Dynamic Binding Capacity 10% per unit
    0.55 mg/well

Product Information

  • Membrane Type
    Strong anion exchange membrane
  • Pack Size
    2
  • Primary Product Type
    Membranes
  • Type
    Membrane adsorber (96-well plate)
  • What you receive
    • 2 plates
    • 4 deep well collection plate 2 ml
    • 1 operating instruction

Specifications

  • Ligand
    Quaternary ammonium
  • Membrane Adsorber Format
    96-Well Plates
  • pH Stability
    pH 2 – 14 (short term)

Usage Protocols

  • Sterilization Method
    Autoclavable

Volumes

  • Bed Volume
    19 µL/well
Vac8 Strip Spare O-Ring
Vac8 Strip Spare O-Ring
Item no.:   VW08VAA04

Vac96 Well Spare O-Ring
Vac96 Well Spare O-Ring
Item no.:   VW96VAA05

The Vac96 vacuum manifold has been designed specifically for use with 96 multiwell plates. The long drip nozzles on the outlet eliminate cross-talk between flow channels.

Vac8 Vacuum Manifold
Vac8 Vacuum Manifold
Item no.:   VW08VAC01

Vacuum filtration manifold for individual Vac 8-strips. Simple and flexible handling for processing of low numbers of samples.

Vac96 Well Trap & Reservoir
Vac96 Well Trap & Reservoir
Item no.:   VW96VAA02

The Vac96 vacuum manifold has been designed specifically for use with 96 multiwell plates. The long drip nozzles on the outlet eliminate cross-talk between flow channels.

Vac96 Collection Plate 2 ml x25
Vac96 Collection Plate 2 ml x25
Item no.:   VW96VAA04

The Vac96 vacuum manifold has been designed specifically for use with 96 multiwell plates. The long drip nozzles on the outlet eliminate cross-talk between flow channels.