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Centrisart I | Centrifugal Ultrafiltration

Centrisart® I is a ready-to-use unit for small volume centrifugal ultrafiltration to separate proteins from low molecular weight substances in biological samples. Centrisart® I features a unique design: Ultrafiltration in the direction opposite to the centrifugal force. This is so effective in preventing premature blockage of the filter that even whole blood samples can be deproteinized. Centrisart® I can be used in fixed-angle or swinging-bucket rotors accepting standard 15 ml tubes. Ultrafiltration of 90% of the original sample volume is typically completed in about 30 min.The ultrafiltrate is collected in the floater insert tube, where it is readily accessible without removing the tube. Removal of proteins from biological samples is a critical step for e.g. drug binding studies or the determination of metabolites in serum.

  • Centrifugal ultrafiltration against direction of centrifugal force
  • Removal of proteins from biological samples, even whole blood
  • Fast and simple preparation of protein-free ultrafiltrates
  • Wide range of MWCO

Typical applications include

  • Drug binding studies
  • Determination of metabolites in serum
  • Protein removal from blood samples

Performance Characteristics

Time to filter
50% of sample
volume
Time to filter
90% of sample
volume
Passage of
sample
species
volume
BSA 1.0 mg/ml (66,000 MW)
5,000 MWCO 300 min. n/a 0 %
10,000 MWCO 35 min. 80 min. 2 %
20,000 MWCO 9 min. 20 min. 2 %
IgG 0.25 mg/ml (160,000 MW)
100,000 MWCO 13 min. 35 min. 3 %
Blue Dextran 0.1 mg/ml (2,000,000 MW)
300,000 MWCO 9 min. 25 min. 28 %

   

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Products (8)

Centrisart® D-16C Refrigerated Benchtop Centrifuge

A compact, refrigerated, bench top centrifuge with good capacity and optional high speed settings of up to 15,300 rpm make it an all purpose machine ideally suited for tissue culture as well as molecular biology applications.

Centrisart® G-16 Benchtop Centrifuge

A high-capacity, low profile, quiet, non-refrigerated , bench top centrifuge with high speed options capable of utilizing multiple and vessel types for use in both low to high throughput applications.

Centrisart® G-16C Refrigerated Benchtop Centrifuge

A high-capacity, refrigerated, low profile, quiet, non-refrigerated , bench top centrifuge with high speed options capable of utilizing multiple and vessel types for use in both low to high throughput applications.

Centrisart® G-26C Refrigerated High-Speed Benchtop Centrifuge

High-speed, refrigerated, yet low profile, bench top centrifuge with multiple rotor and vessel options for high and low throughput and applications in both molecular biology as well as cell culture.

Centrisart® I Centrifugal Ultrafiltration Devices - 5 kDa MWCO (CTA)

$83.00

12 Pieces
MWCO
5 kD
Centrisart® I Centrifugal Ultrafiltration Devices - 10 kDa MWCO (CTA)

$83.00

12 Pieces
MWCO
10 kD
Centrisart® I Centrifugal Ultrafiltration Devices - 300 kDa MWCO (PES)

$83.00

12 Pieces
MWCO
300 kD
Centrisart® I Centrifugal Ultrafiltration Devices - 20 kDa MWCO (CTA)

$83.00

12 Pieces
MWCO
20 kD
Results per page: | |

Centrisart® I is a ready-to-use unit for small volume centrifugal ultrafiltration to separate proteins from low molecular weight substances in biological samples. Centrisart® I features a unique design: Ultrafiltration in the direction opposite to the centrifugal force. This is so effective in preventing premature blockage of the filter that even whole blood samples can be deproteinized. Centrisart® I can be used in fixed-angle or swinging-bucket rotors accepting standard 15 ml tubes. Ultrafiltration of 90% of the original sample volume is typically completed in about 30 min.The ultrafiltrate is collected in the floater insert tube, where it is readily accessible without removing the tube. Removal of proteins from biological samples is a critical step for e.g. drug binding studies or the determination of metabolites in serum.

  • Centrifugal ultrafiltration against direction of centrifugal force
  • Removal of proteins from biological samples, even whole blood
  • Fast and simple preparation of protein-free ultrafiltrates
  • Wide range of MWCO

Typical applications include

  • Drug binding studies
  • Determination of metabolites in serum
  • Protein removal from blood samples

Performance Characteristics

Time to filter
50% of sample
volume
Time to filter
90% of sample
volume
Passage of
sample
species
volume
BSA 1.0 mg/ml (66,000 MW)
5,000 MWCO 300 min. n/a 0 %
10,000 MWCO 35 min. 80 min. 2 %
20,000 MWCO 9 min. 20 min. 2 %
IgG 0.25 mg/ml (160,000 MW)
100,000 MWCO 13 min. 35 min. 3 %
Blue Dextran 0.1 mg/ml (2,000,000 MW)
300,000 MWCO 9 min. 25 min. 28 %

Application Note

High Recovery of Cationised Protein in Centrisart® I
en 2.1 MB

Catalog

Lab Ultrafiltration and Purification Products
en 11.2 MB

Datasheet

Datasheet Service LPS Calibration Centrifuge
en 417.5 kB
Test Protocol Rotational Speed Test Protocol Temperature — Centrisart® Laboratory Centrifuge (US and Canada)
en 411.7 kB
Datasheet Service LPS Installation Centrifuge
en 405.4 kB
Datasheet Service LPS Installation Centrifuge
de 414.0 kB
Datasheet Service LPS IQ-OQ Centrifuge
en 417.6 kB
Datasheet Service LPS IQ-OQ Centrifuge
de 428.6 kB
Datasheet Service LPS Preventative Maintenance Centrifuge
de 414.1 kB
Datasheet Service LPS Preventative Maintenance Centrifuge
en 405.5 kB

User Manual

Centrisart® 1
en 304.1 kB

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