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Vivaspin 500, 300,000 MWCO PES, 25pc

500 µl - 5 µl
One step procedure for small sample amounts.

Item no.:  VS0151

Price:  $120.00

Availability (ex works):  For more information please log in

   

Vivaspin® centrifugal concentrators are disposable ultrafiltration devices for the concentration of biological samples. Vivaspin® 500 centrifugal filter units offer a simple, one step procedure for sample concentration with starting volumes up to 500 µl. They can effectively be used in fixed angle rotors accepting 2.2 ml centrifuge tubes. The patented vertical membrane design and thin channel filtration chamber (US 5,647,990), minimises membrane fouling and provides high speed concentrations. Vivaspin® 500 units are available with PES membrane in a wide range of molecular weight cut-offs. Concentrate recoveries of > 90% can be reached in less than 30 min. The dead stop pocket prevents concentration to dryness.

  • High flux PES membrane
  • High retentate recovery > 90 %
  • Vertical membrane design for ultra fast concentration
  • Universal rotor compatibility
  • Safe sample concentration due to dead-stop volume
  • Convenient concentrate recovery by pipetting
  • Volume graduation for easy reading/monitoring

Vivaspin® 500 Polyethersulfone, 300 kDa

Performance Characteristics

Time to concentrate up to 30x [min.] at 20°C Concentrate recovery %
Start volume 500 μl 500 μl
Aprotinin 0.25 mg/ml (6,500 MW)
3,000 MWCO PES 30 96 %
BSA 1.0 mg/ml (66,000 MW)
5,000 MWCO PES 15 96 %
10,000 MWCO PES 5 96 %
30,000 MWCO PES 5 95 %
IgG 0.25 mg/ml (160,000 MW)
30,000 MWCO PES 10 96 %
50,000 MWCO PES 10 96 %
100,000 MWCO PES 10 96 %

Application Note

Concentration to a defined final volume with Vivaspin® Turbo 15 and Vivaspin 500
en 96.0 kB
Sartorius Ultrafiltration Products in the Preparation of Biological and Medical Nanocarriers – a Short Review
en 133.1 kB
Urine Protein Concentration with Vivaspin® Concentrators
en 540.6 kB
Vivaspin® 500 — High-cell-density Cultivation of Ultrafiltration: a substitute to lyophilization
en 107.6 kB
Desalting and Buffer Exchange with Vivaspin® Centrifugal Concentrators
en 401.4 kB
Treatment of Vivaspin® concentrators for improved recovery of low-concentrated protein samples
en 272.3 kB

Brochure

Ultrafiltration Family
en 1.6 MB
宣传手册:实验室过滤产品精简目录
zh 19.0 MB

Catalog

Laboratory Filtration Products
en 21.8 MB

User Manual

Vivaspin® 500 μl y 2 ml
es 314.3 kB
Vivaspin® 500 μl and 2 ml
en 296.7 kB
Vivaspin® 500 μl und 2 ml
de 314.5 kB
Vivaspin® 500 μl e 2 ml
it 313.7 kB

Applications

  • Application
    Protein Concentration
  • Area of Application
    • Buffer Exchange
    • Molecule/Protein/DNA Concentration
  • Market
    • BioPharma
    • Research
    • Environmental

Certificates

  • Certification
    None

Compliance Information

  • Animal Components
    No
  • Electrical / Battery item
    No

Dimensions

  • Membrane Area
    0.5 cm²
  • Overall Length
    50 mm
  • Width
    11 mm

Materials of Construction

  • Concentrator Body/Sleeve
    Polycarbonate (PC)
  • Concentrator Cap
    Polycarbonate (PC)
  • Filtrate Vessel
    Polypropylene (PP)
  • Membrane
    Polyethersulfone (PES)
  • Membrane Material
    Polyethersulfone (PES)

Physicochemical Information

  • MWCO
    300 kD

Product Information

  • Device Type
    Centrifugal Ultrafiltration Devices
  • Pack Size
    25
  • Primary Product Type
    Ultrafiltration Devices
  • Sample Type
    • Protein
    • Peptide
    • Nanoparticles
    • Virus
    • Endotoxin

Volumes

  • Dead Stop Volume
    5 µL
  • Fixed Angled Rotor - Maximum Capacity
    500 µL
  • Hold-up Volume
    < 5 µL
  • Volume Range
    0.1 – 0.5 mL

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