Vivaspin® 20, 5,000 MWCO PES, 48pc
5 - 20 ml
Ultrafiltration unit with unique twin membrane for sample concentration with high process flexibility: centrifugation or centrifugation of pressurized units, as well as pressure filtration in stationary benchtop mode.
Item no.: VS2012
Vivaspin® 20 centrifugal concentrators offer increased volume flexibility and performance to the Vivaspin® potfolio. Vivaspin® 20 handles up to 20 ml in swing bucket rotors and 14 ml in fixed angle rotors accepting 50 ml centrifuge tubes. Vivaspin® 20 features twin vertical membranes for unparalleled filtration speed and >100-fold concentration. Volume graduation allows monitoring the concentration process and the modified dead stop pocket simplifies pipetting of the final concentrate. For higher process flexibility, Vivaspin® 20 is available with unique accessories and operating methods. For one-step desalting following concentration, a diafiltration cup filled with buffer is can be placed on the concentrator and spun another time to achieve 98% salt removal, instead of two spins to achieve the same result with the traditional refill and re-spin procedure. When an appropriate centrifuge is unavailable, or for single sample processing, for gas pressure filtration, Vivaspin® 20 can be filled with up to 15ml and then pressurised for bench top concentration. For even faster processing, gas pressure filtration can be combined with centrifugation. 'Pressure-fugation' is particularly useful for difficult or viscous samples such as serum, when using a low process temperature, or generally when minimum process time is essential.
High flux PES membrane
High retentate recovery > 90 %
Vertical twin membrane for ultra-fast concentration
Universal rotor compatibility
Safe sample concentration due to dead-stop volume
Convenient concentrate recovery by pipetting
Volume graduation for easy reading/monitoring
100-fold concentration with twin vertical membranes
Additional option for pressurized operation
Please note: Vivaspin® 20 (30 mm x 116 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. Beckman Allegra 25R with TS-5.1-500 swing-out rotor with BUC 5 buckets and 368327 adaptors; Beckman TA-10.250 25° fixed angle rotor with 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus/Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes. These devices are not designed to fit into rotors that only accept round bottom 29 mm x 105 mm tubes, e.g. Sorvall SS34 or Beckmann JA 20. If your rotor accepts only 29 mm x 105 mm round bottom tubes, please use the Vivaspin® Turbo 15, which can be used in either round bottom or conical centrifuge tubes
Application AreaBuffer Exchange
Electrical / Battery itemNo
Membrane Area6.0 cm²
Overall Length124.5 mm*, 117 mm
Materials of Construction
Concentrator Body/SleevePolycarbonate (PC)
Concentrator CapPolypropylene (PP)
Filtrate VesselPolycarbonate (PC)
Membrane MaterialPolyethersulfone (PES)
Pressure Head / Pressure-fuge HeadAcetal / Aluminium
Centrifugal Ultrafiltration Devices
- Pressure Driven Ultrafiltration Devices
- Centrifugal Ultrafiltration Devices
Dead Stop Volume50 µL
Fixed Angled Rotor - Maximum Capacity14 mL
Hold-up Volume< 20 µL
Swing Bucket Rotor20 mL
Volume Range5 – 20 mL
Volume with Pressure Head15 mL
Use in combination with the Air Pressure Controller (VCA002) and 4 mm pneumatic tubing.
Use in combination with Vivaspin® 100 Pressure Head (VCA800) and 4 mm pneumatic tubing.
Use in combination with Air Pressure Controller (VCA002) for pressurized operation of Vivaspin® 20|100.
Use in combination with Vivaspin® 20 Pressure Head (VCA200) and 4 mm pneumatic tubing.
Use for pressurization of Vivaspin® 20 or 100 centrifugal concentrators.
Use in combination with Vivaspin® 20, Charge Valve (VCA005) and Air Pressure Controller (VCA002).
Diafiltration cups for Vivaspin® 20 units. Diafiltration cups allow the gradual exchange of buffer during diafiltration in Vivaspin® 20.