Vivaspin® 20, 10,000 MWCO PES, 12pc

5 - 20 ml
Ultrafiltration unit with unique twin membrane for sample concentration with high process flexibility: centrifugation or centrifugation of pressurized units, as well as pressure filtration in stationary benchtop mode.

Ultrafiltration unit with a MWCO of 10,000 Dalton and a unique twin membrane (polyethersulfone), suitable for starting volumes up to 20 ml for sample concentration with high process flexibility: centrifugation or centrifugation of pressurised units, as well as pressure filtration in stationary benchtop mode.

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*Custom/bulk order quotes are provided within 24 hours of request.

Vivaspin® 20 centrifugal concentrators offer increased volume flexibility and performance to the Vivaspin® potfolio. Vivaspin® 20 handles up to 20 ml in swing bucket rotors and 14 ml in fixed angle rotors accepting 50 ml centrifuge tubes. Vivaspin® 20 features twin vertical membranes for unparalleled filtration speed and >100-fold concentration. Volume graduation allows monitoring the concentration process and the modified dead stop pocket simplifies pipetting of the final concentrate. For higher process flexibility, Vivaspin® 20 is available with unique accessories and operating methods. For one-step desalting following concentration, a diafiltration cup filled with buffer is can be placed on the concentrator and spun another time to achieve 98% salt removal, instead of two spins to achieve the same result with the traditional refill and re-spin procedure. When an appropriate centrifuge is unavailable, or for single sample processing, for gas pressure filtration, Vivaspin® 20 can be filled with up to 15ml and then pressurised for bench top concentration. For even faster processing, gas pressure filtration can be combined with centrifugation. 'Pressure-fugation' is particularly useful for difficult or viscous samples such as serum, when using a low process temperature, or generally when minimum process time is essential.

    High flux PES membrane

    High retentate recovery > 90 %

    Vertical twin membrane for ultra-fast concentration

    Universal rotor compatibility

    Safe sample concentration due to dead-stop volume

    Convenient concentrate recovery by pipetting

    Volume graduation for easy reading/monitoring

    100-fold concentration with twin vertical membranes

    Additional option for pressurized operation

Rotor Compatibility

Please note: Vivaspin® 20 (30 mm x 116 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. Beckman Allegra 25R with TS-5.1-500 swing-out rotor with BUC 5 buckets and 368327 adaptors; Beckman TA-10.250 25° fixed angle rotor with 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus/Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes. These devices are not designed to fit into rotors that only accept round bottom 29 mm x 105 mm tubes, e.g. Sorvall SS34 or Beckmann JA 20. If your rotor accepts only 29 mm x 105 mm round bottom tubes, please use the Vivaspin® Turbo 15, which can be used in either round bottom or conical centrifuge tubes

Application Note






User Manual


  • Application
    Macromolecule Concentration
  • Application Area
    • Concentration
    • Desalting
    • Buffer Exchange
    • Buffer Exchange
  • Market
    • BioPharma
    • Research

Compliance Information

  • Animal Components
  • Certification
    ISO 9001


  • Membrane Area
    6.0 cm²
  • Overall Length
    124.5 mm*, 117 mm

Materials of Construction

  • Concentrator Body/Sleeve
    Polycarbonate (PC)
  • Concentrator Cap
    Polypropylene (PP)
  • Filtrate Vessel
    Polycarbonate (PC)
  • Membrane
    Polyethersulfone (PES)
  • Membrane Material
    Polyethersulfone (PES)

Physicochemical Information

  • MWCO
    10 kDa

Product Information

  • Brand
  • Device Type
    • Centrifugal
    • Gas Pressure
  • Pack Size
  • Sample Type
    • Protein
    • Peptide
    • Nanoparticles
    • Virus
    • Endotoxin


  • Dead Stop Volume
    50 µL
  • Hold-up Volume
    < 20 µL
  • Maximum Capacity, Fixed Angle Rotor
    14 mL
  • Maximum capacity, Swing bucket Rotor
    20 mL
  • Minimum Recirculation Volume
  • Volume Range
    5 – 20 mL
  • Volume with Pressure Head
    15 mL
Vivaspin® 20 Diafiltration Cups, 12pc

Diafiltration cups for Vivaspin® 20 units. Diafiltration cups allow the gradual exchange of buffer during diafiltration in Vivaspin® 20.

Replacement 4 mm Pneumatic Tubing for VCA002


Use in combination with Air Pressure Controller (VCA002) for pressurized operation of Vivaspin® 20|100.

Female Coupling for 4mm Pneumatic Tubing


Use in combination with Vivaspin® 100 Pressure Head (VCA800) and 4 mm pneumatic tubing.

Male Coupling for 4 mm Pneumatic Tubing


Use in combination with the Air Pressure Controller (VCA002) and 4 mm pneumatic tubing.

Air Pressure Controller (APC)


Use for pressurization of Vivaspin® 20 or 100 centrifugal concentrators.

Vivaspin® 20 Pressure Head


Use in combination with Vivaspin® 20, Charge Valve (VCA005) and Air Pressure Controller (VCA002).

Charge Valve for Pressure Head VCA200


Use in combination with Vivaspin® 20 Pressure Head (VCA200) and 4 mm pneumatic tubing.

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