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Vivaspin 2, 2,000 MWCO Hydrosart, 100pc

0.4 - 2 ml
Three membrane alternatives: PES, Hydrosart® regenerated Cellulose, Cellulosetriacetat. Best recovery of the concentrate by reverse-spinning.

Item no.:  VS02H92

Availability:  On Request

   

Vivaspin® concentrators are disposable ultrafiltration devices for the concentration of biological samples. Vivaspin® 2 can effectively be used in swing bucket or fixed angle rotors accepting 15 ml centrifuge tubes. The patented vertical membrane design and thin channel filtration chamber (US 5,647,990) minimizes membrane fouling and provides high speed concentrations, even with particle-laden solutions. Vivaspin® 2 combines the speed of the classic Vivaspin® products with low internal surface and membrane area in order to achieve superior recoveries from very dilute solutions. Available with a choice of PES, Cellulose Triacetate or Hydrosart® membranes, Vivaspin® 2 offers the highest flexibility for process optimization. Another unique feature of Vivaspin® 2 is the choice of directly pipetting the concentrate from the dead stop pocket built into the bottom of the concentrator or alternatively reverse-spinning the concentrate into the recovery cap. Both methods result in near total concentrate recovery.

  • Choice of three different membranes
  • High retentate recovery > 90 %
  • Vertical membrane design for ultra-fast concentration
  • Universal rotor compatibility
  • Safe sample concentration due to dead-stop volume
  • Convenient concentrate recovery by pipetting
  • Optional re-spin for concentrate recovery
  • Volume graduation for easy reading/monitoring

Performance Characteristics

Time to concentrate up to 30x [min.] at 20°C Concentrate recovery %
Start volume 500 μl 500 μl
Aprotinin 0.25 mg/ml (6,500 MW)
3,000 MWCO PES 30 96 %
BSA 1.0 mg/ml (66,000 MW)
5,000 MWCO PES 15 96 %
10,000 MWCO PES 5 96 %
30,000 MWCO PES 5 95 %
IgG 0.25 mg/ml (160,000 MW)
30,000 MWCO PES 10 96 %
50,000 MWCO PES 10 96 %
100,000 MWCO PES 10 96 %

Application Note

Sartorius Ultrafiltration Products in the Preparation of Biological and Medical Nanocarriers – a Short Review
en 944.4 kB
Sample Preparation for Radio Immunoassay with Vivaspin 2 Ultrafiltration Devices
en 53.3 kB
Vivaflow® and Vivaspin® Workflow in Protein Research Laboratories
en 827.9 kB
Urine Protein Concentration with Vivaspin® Concentrators
en 540.6 kB
Desalting and Buffer Exchange with Vivaspin® Centrifugal Concentrators
en 401.4 kB
Treatment of Vivaspin® concentrators for improved recovery of low-concentrated protein samples
en 485.8 kB
Vivaspin® 500 — High-cell-density Cultivation of Ultrafiltration: a substitute to lyophilization
en 107.6 kB

Brochure

Ultrafiltration Family
en 1.6 MB
宣传手册:实验室过滤产品精简目录
zh 19.0 MB

Catalog

Laboratory Filtration Products
en 8.2 MB

 

  • Concentrator Body/Sleeve
    Polycarbonate (PC)
  • Concentrator Cap
    Polycarbonate (PC)
  • Dead Stop Volume
    8 µL
  • Device Type
    Centrifugal Ultrafiltration Devices
  • Filtrate Vessel
    Polycarbonate (PC)
  • Fixed Angled Rotor - Maximum Capacity
    2 mL
  • Hold-up Volume
    < 10 µL
  • MWCO
    2 K
  • Market
    • BioPharma
    • Research
  • Membrane Area
    1.2 cm²
  • Membrane Material
    Hydrosart®
  • Overall Length
    126 mm
  • Pore Size
    N/A
  • Sample Type
    • Protein
    • Peptide
    • Nanoparticles
    • Virus
    • Endotoxin
  • Swing Bucket Rotor
    3 mL
  • Volume Range
    0.4 – 2 mL

 

  • Application Area
    Buffer Exchange

 

  • Animal Components
    No
  • Application
    Protein Concentration
  • CSA Compliant
    No
  • Certification
    None
  • Electrical / Battery item
    No
  • Pack Size
    100

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