Choose your Vivaspin® 2 Ultrafiltration Devices
Vivaspin® concentrators are disposable ultrafiltration devices for the concentration of biological samples. Vivaspin® 2 can effectively be used in swing bucket or fixed angle rotors accepting 15 ml centrifuge tubes. The patented vertical membrane design and thin channel filtration chamber (US 5,647,990) minimizes membrane fouling and provides high speed concentrations, even with particle-laden solutions. Vivaspin® 2 combines the speed of the classic Vivaspin® products with low internal surface and membrane area in order to achieve superior recoveries from very dilute solutions. Available with a choice of PES, Cellulose Triacetate or Hydrosart® membranes, Vivaspin® 2 offers the highest flexibility for process optimization. Another unique feature of Vivaspin® 2 is the choice of directly pipetting the concentrate from the dead stop pocket built into the bottom of the concentrator or alternatively reverse-spinning the concentrate into the recovery cap. Both methods result in near total concentrate recovery.
- Choice of three different membranes
- High retentate recovery > 90 %
- Vertical membrane design for ultra-fast concentration
- Universal rotor compatibility
- Safe sample concentration due to dead-stop volume
- Convenient concentrate recovery by pipetting
- Optional re-spin for concentrate recovery
- Volume graduation for easy reading/monitoring
Performance Characteristics
Time to concentrate up to 30x [min.] at 20°C | Concentrate recovery % | |
---|---|---|
Start volume | 500 μl | 500 μl |
Aprotinin 0.25 mg/ml (6,500 MW) | ||
3,000 MWCO PES | 30 | 96 % |
BSA 1.0 mg/ml (66,000 MW) | ||
5,000 MWCO PES | 15 | 96 % |
10,000 MWCO PES | 5 | 96 % |
30,000 MWCO PES | 5 | 95 % |
IgG 0.25 mg/ml (160,000 MW) | ||
30,000 MWCO PES | 10 | 96 % |
50,000 MWCO PES | 10 | 96 % |
100,000 MWCO PES | 10 | 96 % |
Application Note
Brochure
Catalog
Flyer
Poster
User Manual
Applications
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ApplicationProtein Concentration
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Application AreaBuffer Exchange
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Market
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BioPharma
- Research
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BioPharma
Compliance Information
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Animal ComponentsNo
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CSA CompliantNo
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CertificationNone
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Electrical / Battery itemNo
Dimensions
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Membrane Area1.2 cm²
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Overall Length126 mm
Materials of Construction
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Concentrator Body/SleevePolycarbonate (PC)
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Concentrator CapPolycarbonate (PC)
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Filtrate VesselPolycarbonate (PC)
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Membrane MaterialHydrosart®
Physicochemical Information
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MWCO5 kDa
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Pore SizeN/A
Product Information
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BrandVivaspin® 2
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Device TypeCentrifugal Ultrafiltration Devices
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Pack Size25
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Sample Type
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Protein
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Peptide
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Nanoparticles
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Virus
- Endotoxin
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Protein
Volumes
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Dead Stop Volume8 µL
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Fixed Angled Rotor - Maximum Capacity2 mL
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Hold-up Volume< 10 µL
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Swing Bucket Rotor3 mL
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Volume Range0.4 – 2 mL