Vivaflow® 50 R, 5,000 MWCO Hydrosart

100 - 1000 ml
Vivaflow® 50R is a ready-to-use and reusable laboratory crossflow cassette for concentrating up to 1 l samples. The active membrane area per device is 50 cm². One unit comes with all necessary accessories for running the device with a laboratory pump and a size 16 pump head. For speeding up the concentration, two cassettes can be run simultaneously.

Price (USD):
Item No.: 

*Custom/bulk order quotes are provided within 24 hours of request.

Vivaflow® 50R is a ready-to-use and reusable laboratory crossflow cassette for concentrating up to 1 l samples. Optimally suited for applications like cell culture supernatant concentration, virus concentration and water sample concentration (e.g. environmental samples or water from fish breeding facilities), this economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market.

The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single module with 50 cm2 active membrane area typically concentrates 250 ml by a factor of 20 in less than 30 min. Alternatively, two Vivaflow® 50R modules can be used in parallel for concentration of 1 l in approx. 45 minutes. Near total recovery of the concentrate is achieved with a single final rinse. Vivaflow® 50R can easily be cleaned and re-used several times.

All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package for using a single cassette. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange.

Equipment required

The standard Vivaflow® 50R package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).

  • Plug and play laboratory cross flow cassette
  • Fast and easy protein sample concentration
  • Reusable
  • Concentrates volumes from 0.1 l -1 l
  • Optimal for cell culture supernatant and virus concentration
  • Smallest cross flow cassette with premium Hydrosart® membrane
  • High flow rate and recovery


  • Application
    Protein Concentration
  • Application Area
    Buffer Exchange
  • Market
    • BioPharma
    • Research

Connections (Physical)

  • Fittings
    Nylon (NY)


  • Channel Height
    0.4 mm
  • Channel Width
    7.5 mm
  • Membrane Area
    50 cm²
  • Overall Height
    100 mm
  • Overall Length
    100 mm

General Specifications

  • Pump Flow
    200 – 400 mL/min
  • Tubing
    PVC (medical grade)

Materials of Construction

  • Flow Channel
  • Flow Restrictor
    Polypropylene (PP)
  • Housing Material
  • Material o-ring | seal
    Silicone (SI)
  • Membrane Material
    Hydrosart® (HY)
  • Membrane Support
    Polypropylene (PP)
  • Pressure Indicator
    Polypropylene, SS spring

Operating Conditions

  • Maximum Allowable Working Temperature
    60 °C (140 °F)
  • Maximum Operating Pressure
    4 bar (58 psi)

Physicochemical Information

  • MWCO
    5 kDa
  • Non-recoverable Hold-up
    < 1 mL

Product Information

  • Brand
  • Device Type
    TFF – (Crossflow)
  • Pack Size
  • Sample Type
    • Protein
    • Peptide
    • Nanoparticles
    • Virus
    • Endotoxin


  • Dead Stop Volume
  • Hold-up Volume
    1.7 mL
  • Minimum Recirculation Volume
    10 mL
  • Volume Range
    100 – 1,000 mL
T-Connectors, Size 16, 2pc


T Connectors for Vivaflow® 50 (2 pieces)

T connectors for running 2 stacks of Vivaflow® 50

Peristaltic Pump Drive, 115v


Pumps used for filtration. Choose from a broad range of universal pumps and manual pumps over laboratory vacuum pumps to peristaltic pumps.

Sample and Diafiltration Reservoir, 500 mL


500 ml sample|diafiltration reservoir to be used with Vivaflow® systems. Allows the convenient application of samples and the continuous replacement of buffer.

Peristaltic Load Pump Head, Size 16


Masterflex Easy Load Pump Head - Size 16