Vivaflow® 50 R, 100,000 MWCO Hydrosart
100 - 1000 ml
Vivaflow® 50R is a ready-to-use and reusable laboratory crossflow cassette for concentrating up to 1 l samples. The active membrane area per device is 50 cm². One unit comes with all necessary accessories for running the device with a laboratory pump and a size 16 pump head. For speeding up the concentration, two cassettes can be run simultaneously.
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Vivaflow® 50R is a ready-to-use and reusable laboratory crossflow cassette for concentrating up to 1 l samples. Optimally suited for applications like cell culture supernatant concentration, virus concentration and water sample concentration (e.g. environmental samples or water from fish breeding facilities), this economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market.
The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single module with 50 cm2 active membrane area typically concentrates 250 ml by a factor of 20 in less than 30 min. Alternatively, two Vivaflow® 50R modules can be used in parallel for concentration of 1 l in approx. 45 minutes. Near total recovery of the concentrate is achieved with a single final rinse. Vivaflow® 50R can easily be cleaned and re-used several times.
All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package for using a single cassette. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange.
The standard Vivaflow® 50R package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).
- Plug and play laboratory cross flow cassette
- Fast and easy protein sample concentration
- Concentrates volumes from 0.1 l -1 l
- Optimal for cell culture supernatant and virus concentration
- Smallest cross flow cassette with premium Hydrosart® membrane
- High flow rate and recovery
Application AreaBuffer Exchange
Channel Height0.4 mm
Channel Width7.5 mm
Membrane Area50 cm²
Overall Height100 mm
Overall Length100 mm
Pump Flow200 – 400 mL/min
TubingPVC (medical grade)
Materials of Construction
Flow RestrictorPolypropylene (PP)
Material o-ring | sealSilicone (SI)
Membrane MaterialHydrosart® (HY)
Membrane SupportPolypropylene (PP)
Pressure IndicatorPolypropylene, SS spring
Maximum Allowable Working Temperature60 °C (140 °F)
Maximum Operating Pressure4 bar (58 psi)
Non-recoverable Hold-up< 1 mL
Device TypeTFF – (Crossflow)
Dead Stop VolumeN/A
Hold-up Volume1.7 mL
Minimum Recirculation Volume10 mL
Volume Range100 – 1,000 mL
T Connectors for Vivaflow® 50 (2 pieces)
T connectors for running 2 stacks of Vivaflow® 50
Pumps used for filtration. Choose from a broad range of universal pumps and manual pumps over laboratory vacuum pumps to peristaltic pumps.
500 ml sample|diafiltration reservoir to be used with Vivaflow® systems. Allows the convenient application of samples and the continuous replacement of buffer.
Masterflex Easy Load Pump Head - Size 16