Skip to content Skip to navigation menu

Vivaflow 50, 3,000 MWCO PES, 2pc

100 ml - 3 l

  • Plug and play laboratory cross flow cassette
  • Modular - scalable to 6 units
  • Disposable
  • Optimal for aqueous sample concentration e.g. proteins and nanoparticles
  • High flow rate and recovery

Item no.:  VF05P9

Price:  $177.00

Availability (ex works):  For more information please log in

Vivaflow® 50 is a disposable and ready-to-use crossflow cassette for filtration and concentration of samples from 100 ml to 3 l. The membrane surface per device is 50 cm². One pack contains two devices and comes with all necessary accessories for running two devices with a laboratory pump and a size 16 pump head. For speeding up the concentration and increasing the volume throughput, six cassettes can be connected.

Optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration e.g. in fish breading facilities, this economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market. The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single 50 cm² module typically reduces 500 ml to 15 ml in less than 50 min. Nearly total recovery of the concentrate is achieved with a single rinse. Up to six of the unique interlocking modules can be connected for easy scale up resulting in a capacity of several liters. Vivaflow® 50 is intended for single use. All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange.

Equipment required

The standard Vivaflow® 50 package comes with two cassettes, tubing, flow restrictor, one series interconnector and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).

Running more than two Vivaflow® 50 devices in parallel and series

For speeding up the concentration, as many as six Vivaflow® 50 cassettes can easily be connected using 2 T-connectors for connecting 2 Vivaflow® cassettes at the inlet and at the outlet and series interconnectors for connecting the devices inbetween. A pressure indicator needs to be built in the inlet for optimal control.

  • High flow rate and recovery
  • Clear housing for process monitoring
  • Ready to use single cassettes
  • Easy set up of multiple cassettes
  • Economical and disposable product

Unique in its performance

A single 50cm² module will typically reduce 500 ml to less than 15 ml in under 50 minutes. Less than 10 ml minimum system recirculation for highest concentrations. Less than 1.5 ml non recoverable hold up volume. Near total recoveries achievable with a single 10 ml rinse.

Performance Characteristics

Time to concentrate up to 20x (min.) at 3 bar
inlet pressure, 20°C
Single device Three devices Solute recovery %
250 ml start vol. min. 1 L start vol. min. Direct 10 ml rinse
BSA 1.0 mg/ml (66,000 MW)
5,000 MWCO PES 34 49 96 % > 99 %
10,000 MWCO PES 22 32 94 % > 99 %
30,000 MWCO PES 22 32 92 % 99 %
50,000 MWCO PES 20 29 92 % 98 %
γ Globulins 1.0 mg/ml
100,000 MWCO PES 43 62 92 % 98 %
100,000 MWCO RC 40 58 92 % 98 %
Yeast 1.0 mg/ml (S.Cerevisiae)
0.2 μm PES 33 47 92 % 98 %

Application Note

Sartorius Ultrafiltration Products in the Preparation of Biological and Medical Nanocarriers – a Short Review
en 133.1 kB
The Measurement of Soluble (< 10 kDa) Trace Metals in seawater by the Vivaflow 50
en 269.6 kB

Brochure

宣传手册:无菌检测产品
zh 4.2 MB
Ultrafiltration Family
en 1.6 MB
宣传手册:实验室过滤产品精简目录
zh 19.0 MB

Catalog

Laboratory Filtration Products (US and Canada)
en 9.6 MB

Datasheet

Vivaflow® 50 | 50R | 200 — Unique, “Plug & Play” Laboratory Crossflow Devices
en 677.1 kB

User Manual

Vivaflow 50 | 50R | 200
en 731.4 kB

Applications

  • Application
    Protein Concentration
  • Area of Application
    • Buffer Exchange
    • Molecule/DNA/Concentration
  • Market
    Research

Certificates

  • Certification
    None

Compliance Information

  • Animal Components
    No
  • Electrical / Battery item
    No

Connections (Physical)

  • Fittings
    Nylon (NY)

Dimensions

  • Channel Height
    0.3 mm
  • Channel Width
    15 mm
  • Membrane Area
    50 cm²
  • Overall Height
    84 mm
  • Overall Length
    107 mm
  • Width
    25 mm

Materials of Construction

  • Flow Channel
    TPX (PMP)
  • Flow Restrictor
    Polypropylene (PP)
  • Housing Material
    Polycarbonate (PC)
  • Material o-ring | seal
    Silicone (SI)
  • Membrane Material
    Polyethersulfone (PES)
  • Membrane Support
    TPX (PMP)
  • Pressure Indicator
    Polypropylene, SS spring

Operating Conditions

  • Maximum Allowable Working Temperature
    60 °C (140 °F)
  • Maximum Operating Pressure
    3 bar (44 psi)

Physicochemical Information

  • MWCO
    3 kD
  • Non-recoverable Hold-up
    < 0.5 mL

Product Information

  • Device Type
    Tangential / Crossflow Concentrator
  • Pack Size
    2
  • Primary Product Type
    Ultrafiltration Devices
  • Sample Type
    • Protein
    • Peptide
    • Nanoparticles

Specifications

  • Pump Flow
    200 – 400 mL/min
  • Tubing
    PVC (medical grade)

Volumes

  • Hold-up Volume
    1.5 mL
  • Minimum Recirculation Volume
    < 10 mL
  • Volume Range
    100 – 3,000 mL
500 ml Sample|Diafiltration Reservoir
500 ml Sample|Diafiltration Reservoir

500 ml sample|diafiltration reservoir to be used with Vivaflow® systems. Allows the convenient application of samples and the continuous replacement of buffer.

Item no.:   VFA006

$166.00

Masterflex Easy Load Pump Head - Size 16
Masterflex Easy Load Pump Head - Size 16

Masterflex Easy Load Pump Head - Size 16

Item no.:   VFA012

$475.00

Vivaflow 50 Stand
Vivaflow 50 Stand

Plate for setting up Vivaflow® 50 units

Vivaflow® 50 stand

Item no.:   VFA016

$184.00

Pressure Indicator (1 - 3 bar) for Vivaflow units
Pressure Indicator (1 - 3 bar) for Vivaflow units

Pressure Indicator (1-3 bar)

Pressure indicator (1-3 bar) for Vivaflow® units

Item no.:   VFA020

$37.00

Flow restrictors (6 x 0.6mm, pink)
Flow restrictors (6 x 0.6mm, pink)

Flow restrictor 0.6 mm (6 pieces)

Flow restrictors 6 x 0.6 pink to be used with the Vivaflow® systems

Item no.:   VFA035

$9.00

T-Connectors for running 2 stacks of Vivaflow 50, 2pc
T-Connectors for running 2 stacks of Vivaflow 50, 2pc

T Connectors for Vivaflow® 50 (2 pieces)

T connectors for running 2 stacks of Vivaflow® 50

Item no.:   VFA030

$9.00

Series Interconnectors, 6pc
Series Interconnectors, 6pc

Series Interconnectors (6 pieces)

Series interconnectors for running up to six Vivaflow® 50 units in parallel

Item no.:   VFA031

$19.00

Female Luer fittings, size 16, 10pc
Female Luer fittings, size 16, 10pc

Female Luer fittings Size 16 (10 pieces) to be used with the Vivaflow® systems

Item no.:   VFA032

$9.00

Tubing set for Vivaflow 50
Tubing set for Vivaflow 50

Tubing Set for Vivaflow® 50

Comprises: 2 x 1 m size 16 PVC tubing with inlet fittings, 2 x 50 cm size 16 PVC tubing with 0.6 mm flow restrictors, 1 x series interconnector

Item no.:   VFA034

$73.00

Masterflex Economy Drive Peristaltic Pump 230 V
Masterflex Economy Drive Peristaltic Pump 230 V

Pump for running the Vivaflow-unit

Item no.:   VFP001

$810.00

Masterflex Economy Drive Peristaltic Pump 115 V
Masterflex Economy Drive Peristaltic Pump 115 V

Pumps used for filtration. Choose from a broad range of universal pumps and manual pumps over laboratory vacuum pumps to peristaltic pumps.

Item no.:   VFP002

$920.00

Customers Also Purchased

Sartolab® RF Vacuum Filtration Units 180C3----------E, 0.22 µm Polyethersulfone, 1000 ml

Sartolab® RF Vacuum Filtration Units 180C3----------E, 0.22 µm Polyethersulfone, 1000 ml

Learn More
Vivaflow 200, 5,000 MWCO PES

Vivaflow 200, 5,000 MWCO PES

Learn More
500 ml Sample|Diafiltration Reservoir

500 ml Sample|Diafiltration Reservoir

Learn More
Masterflex Easy Load Pump Head - Size 16

Masterflex Easy Load Pump Head - Size 16

Learn More
Vivaflow 50R|200 complete set of accessories (230 V pump)

Vivaflow 50R|200 complete set of accessories (230 V pump)

Learn More
Vivaspin Turbo 15, 3,000 MWCO PES, 12pc

Vivaspin Turbo 15, 3,000 MWCO PES, 12pc

Learn More
Vivaspin 20, 3,000 MWCO PES, 12pc

Vivaspin 20, 3,000 MWCO PES, 12pc

Learn More