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Vivacell 250 Pressure Head

Use in combination with Vivacell® 250

Item no.:  VCA015

Availability:  On Request

Vivacell® 250 is the device of choice for the concentration of larger biological samples by pressure ultrafiltration and offers numerous advantages when compared to stirred cells. Vivacell® 250 handles a volume range from 50 ml to 250 ml and can - for maximum simplicity - be used in bench top mode or in a refrigerator. Fastest concentration is achieved by use with gentle orbital shaking, at the same time reducing shearing effects. Vivacell® 250 features concentrator modules with vertical membranes in order to minimize fouling. The unique conical dead stop built into the bottom of the membrane insert allows concentration to > 1 ml. Setup of the unit and exchange of membrane inserts are extremely easy, the concentration process can be monitored through an inspection window with volume graduations.

  • Easy to handle
  • High flux PES membrane
  • Vertical membrane design for ultra-fast concentration
  • High retentate recovery > 90 %
  • Unit reusable, only membrane insert needs to be replaced

Need for Speed!

Use free standing on a bench top or in a refrigerator for maximum simplicity, or use on laboratory shaker for fastest concentrations. The unique conical dead stop built into the bottom of the membrane insert allows concentrations to under 1 ml. The gentle vortex action controls membrane polarisation whilst greatly reducing the shear effects typical of stirring mechanisms. Set up or membrane replacement takes just a few seconds. Quick connect fittings and simple screw closure further enhance ease of use.

Performance Characteristics

Time to concentrate 20x min at 20°C 4 bar pressure
100 ml start volume 250 ml start volume
Orbital shake Free standing Solute recovery % Orbital shake Free standing Solute recovery
BSA 1.0 mg/ml (66,000 MW)
5,000 MWCO PES 19 70 98 % 40 140 99 %
10,000 MWCO PES 12 45 97 % 28 100 98 %
30,000 MWCO PES 12 45 96 % 28 100 98 %
γ Globulins 0.25 mg/ml (160,000 MW)
30,000 MWCO PES 25 120 96 % 55 240 98 %
50,000 MWCO PES 25 120 94 % 55 240 98 %
100,000 MWCO PES 25 120 96 % 58 240 98 %

Brochure

Ultrafiltration Family
en 1.6 MB

Catalog

Laboratory Filtration Products (US and Canada)
en 9.6 MB

User Manual

Vivacell 100
en 194.0 kB

Product properties

  • Width
    116 mm
  • Max. operating pressure
    4 bar
  • Primary Product Type
    Pressure Heads
  • Membrane Material
    Polyethersulfone (PES)

Technical attributes

  • Electrical / Battery item
    No
  • Animal Components
    No
  • Pressure Head / Pressure-fuge Head
    Acetal
  • Concentrator Body/Sleeve
    Polycarbonate (PC)
  • Maximum Pressure
    4 bar
  • Hold-up volume
    < 200 µL
  • Active Membrane Area
    40 cm²
  • Overall height
    235 mm
  • Hold-up Vol. memb. & Support
    < 200 µl
  • Height (inc. Pressure Indicator)
    235 mm
  • Filtrate Container
    Polycarbonate (PC)
  • Quick Release Connector
    Acetal