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Sartobind® IDA 75

Syringe filter unit containing several layers of Sartobind Membrane Adsorber modified with IDA for rapid purification of His-tagged proteins. Binding capacity of 7.5 mg protein per unit. Operated using a peristaltic pump.

Item no.:  93IDA-42DB-12--V

Availability (ex works):  On Request

   

Sartobind® Membrane Adsorbers (MA) for affinity chromatography using metal chelate interaction are ready-to-use syringe filter type units. They are based on the patented Sartobind® membrane that displays a microporous structure with pore size of 0.45 µm. Iminodiacetic acid (IDA) as chelating functional group is bound covalently to the complete internal surface of the membrane, resulting in a separation medium of high binding capacity combined with very high flow rates. Binding of metal ions such as Ni2+ or Co2+ is very stable and allows many cycles of re-use without loss of binding capacity. Sartobind® MA IDA units for affinity chromatography are available with 75 cm² membrane area, corresponding to a static binding capacity of up to 7.5 mg of his-tagged proteins per unit. Sartobind® MA IDA units are equipped with standard Luer Lock connectors and can be run with HPLC or FPLC systems or a pump. Due to their polysulfone housing, they are compatible with many solvents applied in chromatography and can be re-used > 100 times. Sartobind® MA IDA units are the ideal tool for rapid affinity purification of all kinds of proteins. Their membrane structure with large pore size allows separations ten times faster than with conventional columns using beaded media.

  • Ready-to-use devices
  • Simple handling with pumps
  • High binding capacity
  • Very high flow rate
  • Robust separations

Application Note

Purification of His6-tagged Proteins — Metal Chelate Chromatography with Sartobind® Membrane Adsorbers
en 48.5 kB

Brochure

Sartobind® Membrane Chromatography
en 2.3 MB

Datasheet

Sartobind® IDA 75
en 80.0 kB

User Manual

Directions for Use - Sartobind® IDA 75
en 236.5 kB

 

  • Filter Diameter
    ⌀ 25 mm

 

  • Pack Size
    2

 

  • Housing Material
    Polypropylene (PP)
  • Membrane Area
    75 cm²
  • Membrane Material
    Stabilised reinforced cellulose
  • Membrane Type
    Metal chelate membrane
  • Recommended Chelating Ions
    Ni²+, Co²+, Cu²+ or Zn²+
  • Reinforcement Material
    Polyester
  • Typical Dynamic Binding Capacity 10% per unit
    7.5 mg
  • Void Volume
    1.3 mL

 

  • Bed Height
    4 mm
  • Bed Volume
    2.1 mL
  • Chemical Compatibility
    Stable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
  • Ligand
    Iminodiacetic acid
  • Ligand Density
    > 5 µeq/cm²
  • Maximum Operating Pressure
    0.6 MPa
  • Pore Size
    3 - 5 µm
  • Recommended Flow Rate
    10-63 mL/min
  • Reference Protein
    Polyhistidine tagged protein
  • What you receive
    • 2 units
    • 1 operating and instruction sheet
  • pH Stability
    pH 1 – 12 (short term), pH 3 – 9 (long term)