Sartobind® IDA 75
Choose your Sartobind® Lab Units (not scalable)
Sartobind® Membrane Adsorbers (MA) for affinity chromatography using metal chelate interaction are ready-to-use syringe filter type units. They are based on the patented Sartobind® membrane that displays a microporous structure with pore size of 0.45 µm. Iminodiacetic acid (IDA) as chelating functional group is bound covalently to the complete internal surface of the membrane, resulting in a separation medium of high binding capacity combined with very high flow rates. Binding of metal ions such as Ni2+ or Co2+ is very stable and allows many cycles of re-use without loss of binding capacity. Sartobind® MA IDA units for affinity chromatography are available with 75 cm² membrane area, corresponding to a static binding capacity of up to 7.5 mg of his-tagged proteins per unit. Sartobind® MA IDA units are equipped with standard Luer Lock connectors and can be run with HPLC or FPLC systems or a pump. Due to their polysulfone housing, they are compatible with many solvents applied in chromatography and can be re-used > 100 times. Sartobind® MA IDA units are the ideal tool for rapid affinity purification of all kinds of proteins. Their membrane structure with large pore size allows separations ten times faster than with conventional columns using beaded media.
- Ready-to-use devices
- Simple handling with pumps
- High binding capacity
- Very high flow rate
- Robust separations
Application Note
Brochure
Datasheet
User Manual
Dimensions
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Bed Height4 mm
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Filter Diameter⌀ 25 mm
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Membrane Area75 cm²
Features
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ReusableYes
Materials of Construction
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Housing MaterialPolypropylene (PP)
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Membrane MaterialStabilised reinforced cellulose
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Reinforcement MaterialPolyester
Operating Conditions
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Maximum Operating Pressure0.6 MPa
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Recommended Flow Rate10-63 mL/min
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pH StabilitypH 1 – 12 (short term), pH 3 – 9 (long term)
Physicochemical Information
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Bed Volume2.1 mL
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Chemical CompatibilityStable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
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LigandIminodiacetic acid
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Ligand Density> 5 µeq/cm²
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Pore Size3 - 5 µm
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Recommended Chelating IonsNi²+, Co²+, Cu²+ or Zn²+
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Reference ProteinPolyhistidine tagged protein
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Typical Dynamic Binding Capacity 10% per unit7.5 mg
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Void Volume1.3 mL
Product Information
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BrandSartobind®
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Membrane TypeMetal chelate membrane
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Pack Size2
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What you receive
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2 units
- 1 operating and instruction sheet
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2 units