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NutriFreez® D10 Cryopreservation Medium

A chemically defined, animal component-free, protein-free cryopreservation solution.

Item no.:  05-713-1A

   

NutriFreez® D10 Cryopreservation Medium is a ready-to-use, animal component-free, serum-free, and protein-free cell freezing solution optimized for multiple cell lines.  When culturing cells in a serum-free environment, it is essential to also maintain serum-free conditions during cryopreservation.  

NutriFreez® D10 Cryopreservation Medium has been demonstrated to result in high rates of cell viability, proliferation, adherence (in relevant lines), and bioactivity/ expression following freezing and thawing.  Superior results were obtained both in comparison with serum-containing freezing media as well as competing serum-free products, making this an ideal product for both serum-containing and serum-free applications.

NutriFreez® D10 Cryopreservation Medium has been extensively tested on multiple cell types, including:

  • Human Mesenchymal Stem Cells (hMSC), from various sources (e.g. bone marrow (BM-MSC), adipose tissue (AT-MSC), umbilical cord tissue (UC-MSC) derived and dental pulp tissue (hMSC –DP)
  • Human Embryonic Stem Cells (hESC), Induced Pluripotent Stem Cells (iPSC)
  • Human Peripheral Blood Mononuclear Cells (PBMC’s)
  • Human Endothelial Cells (EC)
  • T cells, including Chimeric Antigen Receptors (CART) cells and Tumor-Infiltrating Lymphocytes (TIL’s)
  • Neuron Cells
  • Hybridomas
  • CHO cells
  • Vero Cells
  • Multiple mammalian cell lines: MRC-5, HEK-293, HepG2, HeLaBSC-1, BGM3T3, MA-10BHK-21 as well as other extremely sensitive cell types

 

NutriFreez® D10 Cryopreservation Medium Features

  • Cryoprotective formulation designed to minimize dehydration effects
  • Effective maintenance of cell viability, adhesion, and bioactivity
  • Ready-to-use solution, simple protocol
  • Suitable for use in cell banks and biopharmaceutical applications
  • Animal component-free, serum-free, protein-free solution
  • cGMP Manufactured
  • Drug Master File available

Results of a cryopreservation comparison performed at the Research and Development Center for Cell Therapy (Japan) on human pluripotent stem cells (hESC and hiPSC) show that NutriFreez® D10 Cryopreservation Medium* presents best recovery rate for hPSCs after thawing (Nishishita et al. Am J Stem Cells. 2015).

Furthermore, an evaluation of different cryopreservation agents for mesenchymal stem cells (MSC) performed at Ottawa Hospital Research Institute (Canda) shows that MSC cryopreserved in NutriFreez® D10 Cryopreservation Medium exhibit the best post-thaw viability (Salkhordeh et. al. Cytotherapy, May 2018).

* NutriFreez® brand replaces the brand CryoStem™

Brochure

NutriFreez® D10 Cryopreservation Medium - Discover Performance
en 3.5 MB

Datasheet

NutriFreez® D10 Cryopreservation Medium
en 284.3 kB

Publication

NutriFreez® D10 Cryopreservation Selected References
en 110.9 kB

Safety Datasheet

NutriFreez® D10 Cryopreservation Medium MSDS (GB)
en 119.0 kB
NutriFreez® D10 Cryopreservation Medium MSDS (US)
en 123.6 kB

User Manual

NutriFreez® D10 Cryopreservation Medium Instructions For Use
en 388.6 kB

Compliance Information

  • Origin
    Animal-Component Free

Volumes

  • Volume
    500 ML

eShop Dynamic Attributes

  • Adherence/Suspension
    Adherent/Suspension
  • Application Area
    Cryopreservation of cells
  • Delivery Condition
    Sterile
  • Phenol Red Indicator
    With Phenol Red
  • Serum Free
    Yes

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