MSCgo™ Osteogenic Differentiation Medium is a serum-free (SF), xeno-free (XF), complete, and ready to use formulation developed for optimal differentiation of human mesenchymal stem cells (hMSC) to mature osteocytes. The MSCgo™ Osteogenic Differentiation Medium is validated to efficiently differeniate hMSC from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC), and umbilical cord tissue (UC-MSC).
The MSCgo™ Osteogenic Differentiation protocol is part of a complete system for multipotency evaluation of hMSCs. The MSCgo™ Osteogenic Differentiation Medium enables reliable osteogenesis if hMSCs. Differentiation to mature osteocytes is seen between 14 and 21 days.
- Serum-free, xeno-free solution
- Complete differentiation medium
- Ready-to-use, simple protocol
- Reliable differentiation to mature osteocytes
- Contains stable L-alanyl-L-glutamine
- Does not contain antibiotics
Osteogenic differentiation of hMSC results in the formation of mineralized culture with calcified nodules and calcium secretion that can be detected with Alizarin Red S (ARS) staining. The ARS is used to stain calcium deposits formation which are an indication of mature osteocytes. The amount of calcified nodules formation and calcium secretion can be varying using different hMSC (e.g. source, age, and passage number).
Application AreaOsteogenic Differentiation
Phenol Red IndicatorWith Phenol Red