DMEM/F-12, HEPES

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and fibroblasts.

This DMEM/F-12 is modified as follows:

With

• Sodium Bicarbonate
• Sodium Pyruvate
• HEPES

Without

• L-Glutamine