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MEM - Minimum Essential Media

Minimum Essential Medium (MEM) was developed by Harry Eagle and is one of the most widely used medias of all synthetic cell culture media. MEM is simply a modified Basal Medium Eagle media containing higher concentrations of essential nutrients. MEM has been utilized for the cultivation of a variety of cells grown in monolayers. The optional supplementation of non-essential amino acids (NEAA) to the formulations that incorporate either Hanks’ or Earle's salts has broadened the usefulness of this medium.

   

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MEM-NEAA, Earle's salts, non-essential amino acids

MEM-Eagle with Non-Essential Amino Acids (MEM-NEAA), without L-Glutamine