500 µl - 5 µl
One step procedure for small sample amounts.
Ultrafiltration unit with a MWCO of 10,000 Dalton, suitable for starting volumes up to 500 µl. The Vivaspin® centrifugal concentrator is a disposable ultrafiltration device for sample concentration and features a polyethersulfone (PES) membrane.
Vivaspin® centrifugal concentrators are disposable ultrafiltration devices for the concentration of biological samples. Vivaspin® 500 centrifugal filter units offer a simple, one step procedure for sample concentration with starting volumes up to 500 µl. They can effectively be used in fixed angle rotors accepting 2.2 ml centrifuge tubes. The patented vertical membrane design and thin channel filtration chamber (US 5,647,990), minimises membrane fouling and provides high speed concentrations. Vivaspin® 500 units are available with PES membrane in a wide range of molecular weight cut-offs. Concentrate recoveries of > 90% can be reached in less than 30 min. The dead stop pocket prevents concentration to dryness.
- High flux PES membrane
- High retentate recovery > 90 %
- Vertical membrane design for ultra fast concentration
- Universal rotor compatibility
- Safe sample concentration due to dead-stop volume
- Convenient concentrate recovery by pipetting
- Volume graduation for easy reading/monitoring
Vivaspin® 500 Polyethersulfone, 10 kDa
|Time to concentrate up to 30x [min.] at 20°C||Concentrate recovery %|
|Start volume||500 μl||500 μl|
|Aprotinin 0.25 mg/ml (6,500 MW)|
|3,000 MWCO PES||30||96 %|
|BSA 1.0 mg/ml (66,000 MW)|
|5,000 MWCO PES||15||96 %|
|10,000 MWCO PES||5||96 %|
|30,000 MWCO PES||5||95 %|
|IgG 0.25 mg/ml (160,000 MW)|
|30,000 MWCO PES||10||96 %|
|50,000 MWCO PES||10||96 %|
|100,000 MWCO PES||10||96 %|