text.skipToContent text.skipToNavigation

Sartobind STIC PA® 96-Well Plates

Sartobind STIC® PA (primary amine) anion exchange membrane overcomes reduced binding capacity for host cell protein removal and can be used in high-salt conditions.

Order Number:  99STPA42GC-----V

Availability:  On Request

Sartobind STIC® PA removes contaminants at high salt. Salt-tolerant interaction chromatography (STIC) with PA ligand is based on anion-exchange chromatography (AEX) principles. AEX is the established method for removing process-derived contaminants such as host-cell proteins (HCP), DNA, endotoxins, and adventitious and endogenous viruses. It is performed during the downstream processing of monoclonal antibodies (mAbs) in flow-through mode. The process is driven by throughput and not by mass, and thus membrane chromatography (MC) is advantageous over columns for contaminant removal, as membranes run up to two orders of magnitude higher flow rates. Membranes are easy to operate and can be disposed of after one use, just like filters, to save on validation cost.

Applications:

Current mAb downstream processes consist of at least one initial capture and one AEX chromatography step. The limitation of AEX steps is often that the Q chemistry requires low-salt conditions. In processes with cation exchange (CEX) as the second chromatography step, the CEX pool needs to be diluted to conductivities of 4-7 mS/cm to reestablish binding conditions for the following AEX step. The Sartobind STIC PA membrane is composed of cross-linked, regenerated macro-porous cellulose. The primary amine ligand is attached to the matrix at approximately six-fold higher ligand density than that of Q membrane. Both the free amine and the high ligand density were crucial for developing its high salt tolerance.

Benefits:

The Sartobind STIC® adsorber speeds up the production process by high flow rate and opens new opportunities for the purification of therapeutic proteins and vaccines.

  • No dilution of CEX pools necessary
  • Host cell proteins up to 10kg/l (product load/liter membrane)
  • Viruses >4 log
  • DNA at up to 1.5 M NaCl
  • Endotoxins >4 log

Easy to use:

  • Plug and play devices from 0.08 ml to 1.6 L
  • Can be used like a filter
  • Flow rate up to 30 bed volumes/min
  • Disposable after one use

Brochure

Sartobind® Membrane Adsorbers — Chromatography as easy as filtration
en 3.7 MB

Datasheet

Sartobind® 96-well plates — High Throughput Screening with IEX and HIC Membrane Chromatography
en 843.2 kB

Flyer

Sartobind® selection guide
en 1.0 MB

User Manual

Sartobind® 96-well plates — IEX (Q, S, STIC PA) and HIC (Phenyl) in 8-strips — For High Throughput Screening Based on Macroporous Membranes, 0.8 mm Bed Height
en 300.6 kB

Product properties

  • Connector Inlet
    N/A
  • Max. operating pressure
    35 kPa
  • Pore size final-filter
    3 – 5 µm
  • Bed volume
    19 µL/well
  • Bed height
    0.8 mm
  • Membrane area
    0.7 cm²
  • Primary Product Type
    Membranes
  • Type
    Membrane adsorber (96-well plate)
  • Design
    Conventional filter design
  • Application
    Screening
  • Membrane filter material
    Stabilised reinforced cellulose
  • Membrane material
    Stabilised reinforced cellulose
  • Membrane
    Salt tolerant anion exchanger
  • Pack size
    2
  • Filter Format
    96-Well Plates

Technical attributes

  • Dimensions (WxDxH)
    128 × 85.5 × 25 mm
  • Maximum Pressure
    35 kPa
  • Ligand density
    18 – 22 µeq/cm²
  • Typical dynamic binding capacity 10% per unit
    0.95 mg/well
  • Chemical compatibility
    Stable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
  • Reference protein
    Bovine serum albumin
  • Application
    Screening
  • Reusable
    No
  • What you receive
    2 plates` 4 deep well collection plate 2 ml` 1 operating instruction
  • Materials of Construction
    Polyester
  • Housing material
    Polypropylene (PP)
  • Ligand
    Primary amine (PA)
  • Material holding frame
    Polystyrene
  • Material 8-strip
    Polypropylene (PP)
  • Membrane type
    Salt tolerant interaction membrane (Sartobind STIC®)
  • pH stability
    pH 2 – 14 (short term)