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Sartobind® Q Cassette 1.6 L, 8 mm bed height

Sartobind® Q strong ion exchangers are perfect tools for the polishing of monoclonal antibodies for the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins. As size exclusion effects are neglectible, they are suited for purification of viruses such as adeno or lentiviruses displaying one order of magnitude higher dynamic binding capacity.

The cassette design eliminates the 5 L size limitation for membrane adsorbers and offers simple pod-like modular system up to 100 L membrane volume.

Order Number:  98IEXQ42E-P

Availability:  On Request

Sartobind® Q membrane adsorbers display a macro-porous structure with pore size of > 3 µm. Quaternary ammonium ligands are bound covalently to the complete internal surface of the membrane, resulting in separation media of high binding capacity combined with exceptional high flow rates.

Applications virus and VLP capture:

As size exclusion effects are negligible, they are suited for purification of viruses and display one order of magnitude higher dynamic binding capacity compared to resin. Viruses such as adeno- or lentiviruses are captured at high flow rates. The material allows also >100 cycles of re-use without loss of binding capacity.

Benefits capture:

  • High speed purification at 5 - 30 bed volumes per minute
  • No size exclusion effects for large proteins an viruses / VLPs
  • >10fold higher binding capacities compared to conventional resins

Applications flow-through polishing:

Sartobind Q is the perfect tool for the flow-through polishing of valuable proteins such as monoclonal antibodies. The bed volumes can be kept sufficiently small during the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins from such pharmaceutical proteins.

Benefits flow-through polishing:

  • Virus clearance > 6 log
  • DNA below detection limit
  • Host cell protein removal > 99 %
  • Endotoxin removal > 5 log
  • Save 80 % of column costs

Easy to use:

  • Plug and play devices from 0.08 mL to 5 L
  • Can be used like a filter
  • Flow rate up to 30 bed volumes/min
  • Re-usable or disposable

Brochure

Sartobind® Membrane Adsorbers — Chromatography as easy as filtration
en 3.7 MB

Datasheet

Sartobind® Q and S, 4 and 8 mm bed height — Process-Scale Strong Ion Exchange Membrane Adsorbers
en 1.6 MB

User Manual

Sartobind® Q and S Membrane Adsorbers — Void Volume Optimized Capsules With 4 | 8 mm Bed Height
en 1.3 MB

Product properties

  • Connector Inlet
    Sanitary 1 - 1 ½" via Manifold
  • Connectors
    Sanitary 1 - 1 1/2" via Manifold
  • Pore size final-filter
    3 – 5 µm
  • Bed volume
    1.6 L
  • Bed height
    8 mm
  • Flow rate
    8 L/min
  • Membrane area
    5.8 m²
  • Primary Product Type
    Membranes
  • Type
    Membrane adsorber
  • Design
    Cassette design
  • Application
    Capturing and polishing
  • Filter Format
    Cassettes
  • Membrane filter material
    Stabilised reinforced cellulose
  • Membrane material
    Stabilised reinforced cellulose
  • Membrane
    Strong anion exchanger
  • Pack size
    1

Technical attributes

  • Dimensions (WxDxH)
    634 × 49 × 387 mm
  • Maximum Pressure
    0.2 MPa
  • Thermal stability
    Sartobind cassettes with ion exchange membranes cannot be autoclaved, but they can be gamma irradiated.
  • Ligand density
    2 – 5 µeq/cm²
  • Void volume
    2.9 L
  • Typical dynamic binding capacity 10% per unit
    46 g
  • Maximum Venting Pressure
    0.5 bar, 0.05 MPa, 7 psi
  • Chemical compatibility
    Stable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
  • Reference protein
    Bovine serum albumin
  • Application
    Capturing` Polishing
  • Biosafety
    All materials of this filter element meet the requirements of the current USP Biological Reactivity tests <88> for plastics Class VI (Systemic Injection, Intracutaneous and Implantation tests).
  • What you receive
    1 cassette` 1 operating instruction` 1 certificate
  • Materials of Construction
    Polyester
  • Housing material
    Acrylonitrile butadiene styrene (ABS)
  • Ligand
    Quaternary ammonium
  • Membrane type
    Strong anion exchange membrane
  • pH stability
    pH 2 – 14 (short term), pH 2 – 12 (long term)
  • Reusable
    Yes