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Sartobind® Q SingleSep Mini

Sartobind® Q strong ion exchangers are perfect tools for the polishing of monoclonal antibodies for the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins. As size exclusion effects are neglectible, they are suited for purification of viruses such as adeno or lentiviruses displaying one order of magnitude higher dynamic binding capacity.

Order Number:  92IEXQ42D4-SS--A

Availability:  On Request

Sartobind® Q membrane adsorbers display a macro-porous structure with pore size of > 3 µm. Quaternary ammonium ligands are bound covalently to the complete internal surface of the membrane, resulting in separation media of high binding capacity combined with exceptional high flow rates.

Applications virus and VLP capture:

As size exclusion effects are negligible, they are suited for purification of viruses and display one order of magnitude higher dynamic binding capacity compared to resin. Viruses such as adeno- or lentiviruses are captured at high flow rates. The material allows also >100 cycles of re-use without loss of binding capacity.

Benefits capture:

  • High speed purification at 5 - 30 bed volumes per minute
  • No size exclusion effects for large proteins an viruses / VLPs
  • >10fold higher binding capacities compared to conventional resins

Applications flow-through polishing:

Sartobind Q is the perfect tool for the flow-through polishing of valuable proteins such as monoclonal antibodies. The bed volumes can be kept sufficiently small during the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins from such pharmaceutical proteins.

Benefits flow-through polishing:

  • Virus clearance > 6 log
  • DNA below detection limit
  • Host cell protein removal > 99 %
  • Endotoxin removal > 5 log
  • Save 80 % of column costs

Easy to use:

  • Plug and play devices from 0.08 mL to 5 L
  • Can be used like a filter
  • Flow rate up to 30 bed volumes/min
  • Re-usable or disposable

Brochure

Sartobind® Ion Exchange — Membrane Adsorber capsules, 4 mm bed height
en 1.3 MB
Sartobind® — Product Guide on Improved Versions
en 640.3 kB

Datasheet

Sartobind® SingleSep, 4 mm bed height — Process Scale Membrane Adsorbers Ion Exchange
en 599.5 kB

User Manual

Sartobind® IEX SingleSep — pico 0.08 ml | nano 1 ml | mini 7 ml | 5” 70 ml | 10” 180 ml 20” 360 ml | 30” 540 ml | mega 1.62 l
en 1.1 MB

Product properties

  • Connector Inlet
    Sanitary ½ – ¾"
  • Connectors
    Sanitary 1/2 - 3/4"
  • Pore size final-filter
    3 – 5 µm
  • Bed volume
    7 mL
  • Bed height
    4 mm
  • Flow rate
    140 mL/min
  • Membrane area
    250 cm²
  • Primary Product Type
    Membranes
  • Type
    Membrane adsorber
  • Design
    Conventional filter design
  • Application
    Polishing
  • Filter Format
    Capsules
  • Membrane filter material
    Stabilised reinforced cellulose
  • Membrane material
    Stabilised reinforced cellulose
  • Membrane
    Strong anion exchanger
  • Pack size
    4

Technical attributes

  • Dimensions (WxDxH)
    4.5 × 8.7 cm
  • Maximum Pressure
    0.4 MPa
  • Thermal stability
    Sartobind capsules with ion exchange membranes can be autoclaved at 121 °C for 30 min. We recommend one autoclaving cycle only.
  • Ligand density
    2 – 5 µeq/cm²
  • Void volume
    20 mL
  • Typical dynamic binding capacity 10% per unit
    0.2 g
  • Maximum Venting Pressure
    0.5 bar, 0.05 MPa, 7 psi
  • Recommended flow rate
    0.2 L/min
  • Chemical compatibility
    Stable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
  • Reference protein
    Bovine serum albumin
  • Application
    Polishing
  • pH stability
    pH 2 – 14 (short term)
  • Reusable
    No
  • Biosafety
    All materials of this filter element meet the requirements of the current USP Biological Reactivity tests <88> for plastics Class VI (Systemic Injection, Intracutaneous and Implantation tests).
  • What you receive
    4 capsules` 1 operating instruction` 1 certificate
  • Materials of Construction
    Polypropylene, polyester
  • Housing material
    Polypropylene (PP)
  • Ligand
    Quaternary ammonium
  • Membrane type
    Strong anion exchange membrane