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Vivaflow 50, 10,000 MWCO PES, 2pc

100 ml - 3 l

  • Plug and play laboratory cross flow cassette
  • Modular - scalable to 6 units
  • Disposable
  • Optimal for aqueous sample concentration e.g. proteins and nanoparticles
  • High flow rate and recovery

Order Number:  VF05P0

Price:  $169.00

Availability:  In Stock *

*Detailed delivery date provided during check-out

Vivaflow® 50 is a disposable and ready-to-use crossflow cassette for filtration and concentration of samples from 100 ml to 3 l. The membrane surface per device is 50 cm². One pack contains two devices and comes with all necessary accessories for running two devices with a laboratory pump and a size 16 pump head. For speeding up the concentration and increasing the volume throughput, six cassettes can be connected.

Optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration e.g. in fish breading facilities, this economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market. The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single 50 cm² module typically reduces 500 ml to 15 ml in less than 50 min. Nearly total recovery of the concentrate is achieved with a single rinse. Up to six of the unique interlocking modules can be connected for easy scale up resulting in a capacity of several liters. Vivaflow® 50 is intended for single use. All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange.

Vivaflow® 50 modules include filtrate tube

Equipment required

The standard Vivaflow® 50 package comes with two cassettes, tubing, flow restrictor, one series interconnector and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).

Running more than two Vivaflow® 50 devices in parallel and series

For speeding up the concentration, as many as six Vivaflow® 50 cassettes can easily be connected using 2 T-connectors for connecting 2 Vivaflow® cassettes at the inlet and at the outlet and series interconnectors for connecting the devices inbetween. A pressure indicator needs to be built in the inlet for optimal control.

  • High flow rate and recovery
  • Clear housing for process monitoring
  • Ready to use single cassettes
  • Easy set up of multiple cassettes
  • Economical and disposable product

Unique in its performance

A single 50cm² module will typically reduce 500 ml to less than 15 ml in under 50 minutes. Less than 10 ml minimum system recirculation for highest concentrations. Less than 1.5 ml non recoverable hold up volume. Near total recoveries achievable with a single 10 ml rinse.

Performance Characteristics

Time to concentrate up to 20x (min.) at 3 bar
inlet pressure, 20°C
Single device Three devices Solute recovery %
250 ml start vol. min. 1 L start vol. min. Direct 10 ml rinse
BSA 1.0 mg/ml (66,000 MW)
5,000 MWCO PES 34 49 96 % > 99 %
10,000 MWCO PES 22 32 94 % > 99 %
30,000 MWCO PES 22 32 92 % 99 %
50,000 MWCO PES 20 29 92 % 98 %
γ Globulins 1.0 mg/ml
100,000 MWCO PES 43 62 92 % 98 %
100,000 MWCO RC 40 58 92 % 98 %
Yeast 1.0 mg/ml (S.Cerevisiae)
0.2 μm PES 33 47 92 % 98 %

Application Note

Sartorius Ultrafiltration Products in the Preparation of Biological and Medical Nanocarriers – a Short Review
en 133.1 kB
The Measurement of Soluble (< 10 kDa) Trace Metals in seawater by the Vivaflow 50
en 269.6 kB

Brochure

Ultrafiltration Family
en 1.6 MB

Catalog

Laboratory Filtration Products (US and Canada)
en 9.6 MB

Datasheet

Vivaflow® 50 | 50R | 200 — Unique, “Plug & Play” Laboratory Crossflow Devices
en 677.1 kB

User Manual

Vivaflow 50 | 50R | 200
en 731.4 kB

Product properties

  • MWCO
    10,000 Daltons
  • Width
    25 mm
  • Volume Range
    100 – 3,000 mL
  • Max. operating pressure
    3 bar
  • Primary Product Type
    Ultrafiltration Devices
  • Membrane material
    Polyethersulfone (PES)
  • Membrane
    Polyethersulfone (PES)
  • Tubing
    PVC (medical grade)
  • Material o-ring | seal
    Silicone (SI)

Technical attributes

  • Electrical / battery item
    No
  • Animal Components
    No
  • Housing material
    Polycarbonate (PC)
  • Pressure indicator
    Polypropylene, SS spring
  • Flow Channel
    TPX (PMP)
  • Flow Restrictor
    Polypropylene (PP)
  • Maximum Pressure
    3 bar
  • Overall length
    107 mm
  • Hold-up volume
    1.5 mL
  • Active Membrane Area
    50 cm²
  • Overall height
    84 mm
  • Max. temperature
    60° C
  • Non-recoverable Hold-up
    < 0.5 mL
  • Channel width
    15 mm
  • Minimum Recirculation Volume
    < 10 mL
  • Channel height
    0.3 mm
  • Pump Flow
    200 – 400 mL/min
  • Certification
    None
  • Application
    Protein Concentration
  • Area of application
    Buffer Exchange` Protein/DNA Concentration
  • Market
    Research
  • Device type
    Tangential / Crossflow Concentrator
  • Sample Type
    Protein` Peptide` Nanoparticles` Virus
  • Fittings
    Nylon (NY)
  • Membrane support
    TPX (PMP)