Configurate your Vivaspin® 500
Vivaspin® centrifugal concentrators are disposable ultrafiltration devices for the concentration of biological samples. Vivaspin® 500 centrifugal filter units offer a simple, one step procedure for sample concentration with starting volumes up to 500 µl. They can effectively be used in fixed angle rotors accepting 2.2 ml centrifuge tubes. The patented vertical membrane design and thin channel filtration chamber (US 5,647,990), minimises membrane fouling and provides high speed concentrations. Vivaspin® 500 units are available with PES membrane in a wide range of molecular weight cut-offs. Concentrate recoveries of > 90% can be reached in less than 30 min. The dead stop pocket prevents concentration to dryness.
- High flux PES membrane
- High retentate recovery > 90 %
- Vertical membrane design for ultra fast concentration
- Universal rotor compatibility
- Safe sample concentration due to dead-stop volume
- Convenient concentrate recovery by pipetting
- Volume graduation for easy reading/monitoring
Vivaspin® 500 Polyethersulfone, 5 kDa
Performance Characteristics
Time to concentrate up to 30x [min.] at 20°C | Concentrate recovery % | |
---|---|---|
Start volume | 500 μl | 500 μl |
Aprotinin 0.25 mg/ml (6,500 MW) | ||
3,000 MWCO PES | 30 | 96 % |
BSA 1.0 mg/ml (66,000 MW) | ||
5,000 MWCO PES | 15 | 96 % |
10,000 MWCO PES | 5 | 96 % |
30,000 MWCO PES | 5 | 95 % |
IgG 0.25 mg/ml (160,000 MW) | ||
30,000 MWCO PES | 10 | 96 % |
50,000 MWCO PES | 10 | 96 % |
100,000 MWCO PES | 10 | 96 % |
Application Note
Concentration to a defined final volume with Vivaspin® Turbo 15 and Vivaspin 500
Sartorius Ultrafiltration Products in the Preparation of Biological and Medical Nanocarriers – a Short Review
Vivaspin® 500 — High-cell-density Cultivation of Ultrafiltration: a substitute to lyophilization
Desalting and Buffer Exchange with Vivaspin® Centrifugal Concentrators
Treatment of Vivaspin® concentrators for improved recovery of low-concentrated protein samples
Urine Protein Concentration with Vivaspin® Concentrators
Brochure
Ultrafiltration Family
Catalog
Laboratory Filtration Products (US and Canada)
User Manual
Vivaspin 500 μl et 2 ml
Vivaspin® 500 μl y 2 ml
Vivaspin® 500 μl and 2 ml
Vivaspin® 500 μl und 2 ml
Vivaspin® 500 μl e 2 ml
Product properties
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MWCO5,000 Daltons
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Width10 mm
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Volume Range0.1 – 0.5 mL
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Primary Product TypeUltrafiltration Devices
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Membrane materialPolyethersulfone (PES)
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MembranePolyethersulfone (PES)
Technical attributes
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Electrical / battery itemNo
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Animal ComponentsNo
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Concentrator CapPolycarbonate (PC)
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Concentrator Body/ SleevePolycarbonate (PC)
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Overall length50 mm
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Hold-up volume< 5 µL
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Active Membrane Area0.7 cm²
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Dead stop volume5 µL
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Fixed Angle Rotor600 µL
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CertificationNone
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Filtrate VesselPolypropylene (PP)
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ApplicationProtein Concentration
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Area of applicationBuffer Exchange` Protein/DNA Concentration
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MarketBioPharma` Research
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Device typeCentrifugal Ultrafiltration Devices
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Sample TypeProtein` Peptide` Nanoparticles
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Charge valve for Pressure-fuge head for Vivacell 70
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