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Sartobind STIC® PA Cassette 0.8 L, 4 mm bed height

Sartobind STIC® PA (primary amine) anion exchange membrane overcomes reduced binding capacity for host cell protein removal and can be used in high-salt conditions.

The cassette design eliminates the 5 L size limitation for membrane adsorbers and offers simple pod-like modular system up to 100 L membrane volume.

Item no.:  98STPA42D-L

Availability:  On Request

Sartobind STIC® PA removes contaminants at high salt. Salt-tolerant interaction chromatography (STIC) with PA ligand is based on anion-exchange chromatography (AEX) principles. AEX is the established method for removing process-derived contaminants such as host-cell proteins (HCP), DNA, endotoxins, and adventitious and endogenous viruses. It is performed during the downstream processing of monoclonal antibodies (mAbs) in flow-through mode. The process is driven by throughput and not by mass, and thus membrane chromatography (MC) is advantageous over columns for contaminant removal, as membranes run up to two orders of magnitude higher flow rates. Membranes are easy to operate and can be disposed of after one use, just like filters, to save on validation cost.


Current mAb downstream processes consist of at least one initial capture and one AEX chromatography step. The limitation of AEX steps is often that the Q chemistry requires low-salt conditions. In processes with cation exchange (CEX) as the second chromatography step, the CEX pool needs to be diluted to conductivities of 4-7 mS/cm to reestablish binding conditions for the following AEX step. The Sartobind STIC PA membrane is composed of cross-linked, regenerated macro-porous cellulose. The primary amine ligand is attached to the matrix at approximately six-fold higher ligand density than that of Q membrane. Both the free amine and the high ligand density were crucial for developing its high salt tolerance.


The Sartobind STIC® adsorber speeds up the production process by high flow rate and opens new opportunities for the purification of therapeutic proteins and vaccines.

  • No dilution of CEX pools necessary
  • Host cell proteins up to 10kg/l (product load/liter membrane)
  • Viruses >4 log
  • DNA at up to 1.5 M NaCl
  • Endotoxins >4 log

Easy to use:

  • Plug and play devices from 0.08 ml to 1.6 L
  • Can be used like a filter
  • Flow rate up to 30 bed volumes/min
  • Disposable after one use


Sartobind® Membrane Adsorbers — Chromatography as easy as filtration
en 3.7 MB


Sartobind STIC® PA, 4 mm bed height — Salt Tolerant Membrane Adsorbers Anion Exchange
en 1.5 MB


Sartobind® selection guide
en 1.0 MB

User Manual

Void Volume Optimized Capsules and Cassettes With 4 mm Bed Height
en 371.5 kB

Product properties

  • Connectors
    Sanitary 1 - 1 ½" via Manifold
  • Pore size final-filter
    3 – 5 µm
  • Bed volume
    0.8 L
  • Bed height
    4 mm
  • Flow rate
    16 L/min
  • Membrane area
    2.9 m²
  • Primary Product Type
  • Type
    Membrane adsorber
  • Design
    Cassette design
  • Filter Format
  • Membrane Material
    Stabilised reinforced cellulose
  • Membrane
    Salt tolerant anion exchanger
  • Pack Size

Technical attributes

  • Dimensions (WxDxH)
    634 × 49 × 387 mm
  • Maximum Pressure
    0.2 MPa
  • Thermal stability
    Sartobind cassettes with ion exchange membranes cannot be autoclaved, but they can be gamma irradiated.
  • Ligand Density
    18 – 22 µeq/cm²
  • Maximum Venting Pressure
    0.5 bar, 0.05 MPa, 7 psi
  • Chemical Compatibility
    Stable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
  • Reference Protein
    Bovine serum albumin
  • Application
  • Biosafety
    All materials of this filter element meet the requirements of the current USP Biological Reactivity tests for plastics Class VI (Systemic Injection, Intracutaneous and Implantation tests).
  • What you receive
    • 1 cassette
    • 1 operating instruction
    • 1 certificate
  • Materials of Construction
  • Housing Material
    Acrylonitrile butadiene styrene (ABS)
  • Ligand
    Primary amine (PA)
  • Membrane Type
    Salt tolerant interaction membrane (Sartobind STIC®)
  • pH Stability
    pH 2 – 14 (short term), pH 2 – 12 (long term)
  • Reusable
  • Typical Dynamic Binding Capacity 10% per unit
    40.6 g
  • Void Volume
    2.5 L