Sartobind® Q Cassette 1.6 L, 8 mm bed height
Sartobind® Q strong ion exchangers are perfect tools for the polishing of monoclonal antibodies for the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins. As size exclusion effects are neglectible, they are suited for purification of viruses such as adeno or lentiviruses displaying one order of magnitude higher dynamic binding capacity.
The cassette design eliminates the 5 L size limitation for membrane adsorbers and offers simple pod-like modular system up to 100 L membrane volume.
Item no.: 98IEXQ42E-P
Availability (ex works): On Request
Sartobind® Q membrane adsorbers display a macro-porous structure with pore size of > 3 µm. Quaternary ammonium ligands are bound covalently to the complete internal surface of the membrane, resulting in separation media of high binding capacity combined with exceptional high flow rates.
Applications virus and VLP capture:
As size exclusion effects are negligible, they are suited for purification of viruses and display one order of magnitude higher dynamic binding capacity compared to resin. Viruses such as adeno- or lentiviruses are captured at high flow rates. The material allows also >100 cycles of re-use without loss of binding capacity.
- High speed purification at 5 - 30 bed volumes per minute
- No size exclusion effects for large proteins an viruses / VLPs
- >10fold higher binding capacities compared to conventional resins
Applications flow-through polishing:
Sartobind Q is the perfect tool for the flow-through polishing of valuable proteins such as monoclonal antibodies. The bed volumes can be kept sufficiently small during the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins from such pharmaceutical proteins.
Benefits flow-through polishing:
- Virus clearance > 6 log
- DNA below detection limit
- Host cell protein removal > 99 %
- Endotoxin removal > 5 log
- Save 80 % of column costs
Easy to use:
- Plug and play devices from 0.08 mL to 5 L
- Can be used like a filter
- Flow rate up to 30 bed volumes/min
- Re-usable or disposable
Reference ProteinBovine serum albumin
BiosafetyAll materials of this filter element meet the requirements of the current USP Biological Reactivity tests for plastics Class VI (Systemic Injection, Intracutaneous and Implantation tests).
ConnectorsSanitary 1 – 1 ½" via Manifold
Bed Height8 mm
Dimensions (W×D×H)634 × 49 × 387 mm
Membrane Area5.8 m²
Materials of Construction
Housing MaterialAcrylonitrile butadiene styrene (ABS)
Materials of ConstructionPolyester
MembraneStrong anion exchanger
Membrane MaterialStabilised reinforced cellulose
Maximum Pressure0.2 MPa
Maximum Venting Pressure0.5 bar, 0.05 MPa, 7 psi
Thermal StabilitySartobind cassettes with ion exchange membranes cannot be autoclaved, but they can be gamma irradiated.
Chemical CompatibilityStable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
Ligand Density2 – 5 µeq/cm²
Pore Size Final-filter3 – 5 µm
Typical Dynamic Binding Capacity 10% per unit46 g
Membrane TypeStrong anion exchange membrane
Primary Product TypeMembranes
What you receive
1 operating instruction
- 1 certificate
- 1 cassette
Recommended Flow Rate8 L/min
pH StabilitypH 2 – 14 (short term), pH 2 – 12 (long term)
Bed Volume1.6 L
Void Volume2.9 L