Sartobind® Q Cassette 1.6 L, 8 mm bed height
Sartobind® Q strong ion exchangers are perfect tools for the polishing of monoclonal antibodies for the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins. As size exclusion effects are neglectible, they are suited for purification of viruses such as adeno or lentiviruses displaying one order of magnitude higher dynamic binding capacity.
The cassette design eliminates the 5 L size limitation for membrane adsorbers and offers simple pod-like modular system up to 100 L membrane volume.
Item no.: 98IEXQ42E-P
Availability (ex works): On Request
Choose your Sartobind® Q configuration
Sartobind® Q membrane adsorbers display a macro-porous structure with pore size of > 3 µm. Quaternary ammonium ligands are bound covalently to the complete internal surface of the membrane, resulting in separation media of high binding capacity combined with exceptional high flow rates.
Applications virus and VLP capture:
As size exclusion effects are negligible, they are suited for purification of viruses and display one order of magnitude higher dynamic binding capacity compared to resin. Viruses such as adeno- or lentiviruses are captured at high flow rates. The material allows also >100 cycles of re-use without loss of binding capacity.
Benefits capture:
- High speed purification at 5 - 30 bed volumes per minute
- No size exclusion effects for large proteins an viruses / VLPs
- >10fold higher binding capacities compared to conventional resins
Applications flow-through polishing:
Sartobind Q is the perfect tool for the flow-through polishing of valuable proteins such as monoclonal antibodies. The bed volumes can be kept sufficiently small during the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins from such pharmaceutical proteins.
Benefits flow-through polishing:
- Virus clearance > 6 log
- DNA below detection limit
- Host cell protein removal > 99 %
- Endotoxin removal > 5 log
- Save 80 % of column costs
Easy to use:
- Plug and play devices from 0.08 mL to 5 L
- Can be used like a filter
- Flow rate up to 30 bed volumes/min
- Re-usable or disposable
Application Note
Brochure
Datasheet
Flyer
User Manual
Applications
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Application
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Capturing
- Polishing
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Capturing
Biological Information
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Reference ProteinBovine serum albumin
Compliance Information
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BiosafetyAll materials of this filter element meet the requirements of the current USP Biological Reactivity tests for plastics Class VI (Systemic Injection, Intracutaneous and Implantation tests).
Connections Physical
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ConnectorsSanitary 1 – 1 ½" via Manifold
Dimensions
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Bed Height8 mm
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Dimensions (W×D×H)634 × 49 × 387 mm
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Membrane Area5.8 m²
Features
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ReusableYes
Materials of Construction
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Housing MaterialAcrylonitrile butadiene styrene (ABS)
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Materials of ConstructionPolyester
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MembraneStrong anion exchanger
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Membrane MaterialStabilised reinforced cellulose
Operating Conditions
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Maximum Pressure0.2 MPa
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Maximum Venting Pressure0.5 bar, 0.05 MPa, 7 psi
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Thermal StabilitySartobind cassettes with ion exchange membranes cannot be autoclaved, but they can be gamma irradiated.
Physicochemical Information
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Chemical CompatibilityStable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
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Ligand Density2 – 5 µeq/cm²
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Pore Size Final-filter3 – 5 µm
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Typical Dynamic Binding Capacity 10% per unit46 g
Product Information
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Membrane TypeStrong anion exchange membrane
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Pack Size1
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Primary Product TypeMembranes
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TypeMembrane adsorber
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What you receive
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1 cassette
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1 operating instruction
- 1 certificate
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1 cassette
Specifications
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DesignCassette design
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Filter FormatCassettes
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LigandQuaternary ammonium
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Recommended Flow Rate8 L/min
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pH StabilitypH 2 – 14 (short term), pH 2 – 12 (long term)
Usage Protocols
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Sterilization MethodAutoclavable
Volumes
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Bed Volume1.6 L
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Void Volume2.9 L