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Sartobind® Phenyl nano 3 mL

Sartobind® Phenyl accomplishes polishing and capturing ten times faster than do conventional HIC resins. The membrane shows comparable, high binding capacity, without size exclusion effects, for proteins in a range of 20 mg mAb/mL membrane, as well as excellent resolution and impurity reduction.

Item no.:  96HICP42EUC11--A

Availability (ex works):  On Request

Hydrophobic interaction chromatography (HIC) separates and purifies biomolecules based on differences in their hydrophobicity. The phenyl membrane adsorber follows the same rules known from the conventional hydrophobic interaction chromatography. Due to the large pore size, membrane adsorbers show excellent flow properties. There is almost no diffusion limitation of mass transport compared with conventional bead chromatography. Buffers with high concentrations of salt promote the adsorption of proteins on the hydrophobic membrane matrix. Proteins are eluted by decreasing the salt concentration in the elution buffer.

The salt dependence of the membrane is comparable to that of columns with ammonium sulfate, sodium chloride and sodium citrate.

Applications:

HIC is a powerful method for differentiating target protein and hydrophobic contaminants. Large proteins bind more strongly than do small ones and aggregates even considerably better than monomers due to multipoint attachment.

Buffer with a high ionic strength, usually ammonium sulfate, is initially added to the protein solution. Salt ions in this solution reduce the solvation of solutes in the sample. As solvation decreases, hydrophobic regions that become exposed are adsorbed by the hydrophobic membrane matrix. In the HIC membrane, flexible cellulose is used as a backbone for the phenyl ligand. The membrane can be regenerated by using either water or alcohol.

The HIC membrane is used for the removal of:

  • Aggregates
  • Host cell proteins
  • Viruses
  • Endotoxins
  • Lipids, dyes, detergents and anti foam agents
  • Leached chromatography ligands

The Phenyl membrane can be used also for binding large proteins such as:

  • Monoclonal antibodies
  • Conjugated vaccines, viruses and phages
  • Oligonucleotides

Benefits:

HIC membranes offer the following advantages over conventional HIC columns:

  • Excellent reduction of aggregates
  • 85 % buffer savings
  • 75 % of time saved

Easy to use:

  • Plug and play devices from 0.08 ml to 5 l
  • Can be used like a filter
  • Flow rate up to 5 bed volumes/min
  • Re-usable >100 cycles or disposable

Application Note

Phenyl Membrane Adsorber for Bioprocessing
en 1.8 MB

Brochure

Sartobind® Membrane Adsorbers — Chromatography as easy as filtration
en 3.7 MB
宣传手册:实验室过滤产品精简目录
zh 19.0 MB

Datasheet

Sartobind® Phenyl, 8 mm bed height
en 1.5 MB

Flyer

Sartobind® selection guide
en 1.0 MB

User Manual

Void Volume Optimized Capsules and Cassettes With 8 mm Bed Height
en 1.3 MB

Applications

  • Application
    • Capturing
    • Polishing

Biological Information

  • Reference Protein
    Gamma globulin from bovine blood

Compliance Information

  • Biosafety
    All materials of this filter element meet the requirements of the current USP Biological Reactivity tests for plastics Class VI (Systemic Injection, Intracutaneous and Implantation tests).

Connections Physical

  • Connectors
    Luer female

Dimensions

  • Bed Height
    8 mm
  • Dimensions (W×D×H)
    3.3 × 3.7 cm
  • Membrane Area
    110 cm²

Features

  • Reusable
    Yes

Materials of Construction

  • Housing Material
    Polypropylene (PP)
  • Materials of Construction
    Polypropylene, polyester
  • Membrane Material
    Stabilised reinforced cellulose

Operating Conditions

  • Maximum Pressure
    0.4 MPa
  • Maximum Venting Pressure
    0.5 bar, 0.05 MPa, 7 psi
  • Thermal Stability
    For Sartobind phenyl devices, pretreatment with sodium hydroxide is recommended instead of autoclaving.

Physicochemical Information

  • Chemical Compatibility
    Stable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
  • Ligand Density
    3 µeq/cm²
  • Pore Size Final-filter
    3 – 5 µm
  • Typical Dynamic Binding Capacity 10% per unit
    44 mg

Product Information

  • Membrane Type
    Hydrophobic interaction chromatography membrane (HIC)
  • Pack Size
    4
  • Primary Product Type
    Membranes
  • Type
    Membrane adsorber
  • What you receive
    • 4 capsules
    • 2 adapters Luer male to UNF 10-32 female
    • 1 operating instruction
    • 1 certificate

Specifications

  • Design
    Optimized design
  • Filter Format
    Capsules
  • Flow Rate
    15 mL/min
  • Ligand
    Phenyl
  • Recommended Flow Rate
    15 mL/min
  • pH Stability
    pH 2 – 14 (short term), pH 3 – 13 (long term)

Volumes

  • Bed Volume
    3 mL
  • Void Volume
    4 mL